Skip to main content
Addgene
Showing: 161 - 180 of 674 results

  1. pT2iD6Hoxa13aER4(#685)

    Plasmid
    #184100
    Purpose
    cyclofen-inducible fish Hoxa13a activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    ZfHoxa13a-ERT2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184100-plasmid-map-sequence-id-364020

    Enlarge

  2. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid
    #126479
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Inserts
    Or56a (Or56a Fly)
    Orco (Orco Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126479-plasmid-map-sequence-id-246521

    Enlarge

  4. pcDNA3.1-E-Flag

    Plasmid
    #101858
    Purpose
    Evi-1 flag tagged for transfection into mammalian cells
    Depositor
    Archibald Perkins
    Insert
    Evi-1 (Mecom Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101858-plasmid-map-sequence-id-211082

    Enlarge

  5. pT2iC6Dronpa2nlsER4(#636)

    Plasmid
    #184067
    Purpose
    cyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Dronpa2-2nls-ERT2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184067-plasmid-map-sequence-id-363960

    Enlarge

  6. pT2iC6FAST1nls2ER4(#638)

    Plasmid
    #184068
    Purpose
    cyclofen-inducible YFAST nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    YFAST-2nls-ERT2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    184068-plasmid-map-sequence-id-365820

    Enlarge

  7. Clim Act MCH (CAM)

    Plasmid
    #104443
    Purpose
    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    actin
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104443-plasmid-map-sequence-id-200869

    Enlarge

  8. Clim EF1 MCH (CEM)

    Plasmid
    #104444
    Purpose
    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    EF1
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104444-plasmid-map-sequence-id-200864

    Enlarge

  9. Clim Tub MCH (CTM)

    Plasmid
    #104445
    Purpose
    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    tubulin promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104445-plasmid-map-sequence-id-200865

    Enlarge

  11. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

    Enlarge

  12. pcDNA3.1-ME-Flag

    Plasmid
    #101860
    Purpose
    Mds1-Evi-1 flag tagged for transfection into mammalian cells
    Depositor
    Archibald Perkins
    Insert
    Mds1-Evi-1 (Mecom Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101860-plasmid-map-sequence-id-211085

    Enlarge

  13. pCSThaP6ERT(#184)

    Plasmid
    #184103
    Purpose
    cyclofen-inducible mouse Pax6 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    HA-MmPax6-ERT2
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6+T7
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184103-plasmid-map-sequence-id-365942

    Enlarge

  14. pCSKaedenERT(#100)

    Plasmid
    #184064
    Purpose
    cyclofen-inducible Kaede nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Kaede-nls-ERT2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184064-plasmid-map-sequence-id-365948

    Enlarge

  15. pCSTG4FER3(#190)

    Plasmid
    #184069
    Purpose
    cyclofen-inducible GAL4 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Gal4bdFF-ERT2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184069-plasmid-map-sequence-id-365692

    Enlarge

  16. pDmelOR-mRho.V5.mER.hOr47a

    Plasmid
    #126478
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Inserts
    Or47a (Or47a Fly)
    Orco (Orco Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126478-plasmid-map-sequence-id-246565

    Enlarge

  17. pCAG-GluA1-scFV-Halo

    Plasmid
    #230065
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230065-plasmid-map-sequence-id-460116

    Enlarge

  19. pCSmp38ERT(#143)

    Plasmid
    #184077
    Purpose
    cyclofen-inducible P38 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-ZfP38a-ERT2
    Use
    Synthetic Biology
    Tags
    5xMyc
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184077-plasmid-map-sequence-id-365706

    Enlarge

  20. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid
    #230055
    Purpose
    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230055-plasmid-map-sequence-id-457173

    Enlarge

Showing: 161 - 180 of 674 results