Showing: 161 - 180 of 591 results
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Plasmid#149394PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation10bp inserted upstream and downstream of the IHFp…PromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Mut
Plasmid#149396PurposeP. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHFDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHFprox site is mutated at key positions needed f…PromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_motif_scram
Plasmid#149397PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationUpstream motif recognized by Cas1-2/3 during spac…PromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pSH124-ssr
Plasmid#198025PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Ap/Cb resistance cassette
UseTagsExpressionBacterialMutationPromoterApR promoterAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE
Plasmid#218873PurposeAAV-mediated expression of improved GABA sensor control (no change in fluorescence)DepositorInsertiGABASnFR2(no bind)
UseAAVTagsExpressionMammalianMutationS99A F102G R168PPromoterGFAPAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-iGABASnFR2(no bind)-WPRE
Plasmid#218876PurposeAAV-mediated expression of improved GABA sensor control (no change in fluorescence)DepositorInsertiGABASnFR2(no bind)
UseAAVTagsExpressionMammalianMutationS99A F102G R168PPromoterSynapsinAvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Tp
Plasmid#65024PurposeTpR mini-Tn7 vector, mobilizableDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pGP-AAV-syn-flex-iGABASnFR2-WPRE
Plasmid#218877PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterSynapsinAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-flex-iGABASnFR2n-WPRE
Plasmid#218878PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F104H R168PPromoterSynapsinAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-flex-iGABASnFR2-WPRE
Plasmid#218880PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-flex-iGABASnFR2n-WPRE
Plasmid#218881PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pCas1_Cas2/3
Plasmid#89240PurposePlasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1DepositorInsertCas1 and Cas2/3
UseTagsStrep II tag on Cas1ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfpC
Plasmid#111616PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfp(ASV)C
Plasmid#111617PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSG019
Plasmid#126502PurposePseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHisDepositorInsertdisaggregase ClpG with 6xHis-tag
UseTagsC-terminal 6xHisExpressionBacterialMutationPromoterPBADAvailabilityAcademic Institutions and Nonprofits only -
pSG020
Plasmid#126503PurposePseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHisDepositorInsertdisaggregase ClpGgi with 6xHis-tag
UseTagsC-terminal 6xHisExpressionBacterialMutationPromoterPBADAvailabilityAcademic Institutions and Nonprofits only -
pQE81L-KOFP7
Plasmid#160330PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.DepositorInsertKOFP7
UseTags6xHis tagExpressionBacterialMutationPromoterT5AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-mcBEST
Plasmid#209416PurposePlasmid for multiplexed cytosine base editing in streptomycetesDepositorInsertsCodon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterbase editing cassette: PtipA ; sgRNAs: PkasO* an…AvailabilityAcademic Institutions and Nonprofits only -
pET15b-Opt12
Plasmid#61697PurposeExpresses PTDH mutant Opt12DepositorInsertOpt12
UseTags6xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 161 - 180 of 591 results