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Plasmid#128591PurposeExpresses tagless S3 ribosomal protein in E.coliDepositorInsertS3 E.coli ribosomal protein (rpsC E.coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI
Plasmid#162803PurposeControl STELLA destination plasmid for N-terminally labelled proteinsDepositorInsertUb(G75,76A)-BsmBI
UsePiggybacTagsExpressionMammalianMutationUbiquitin (G75,76A)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*SMIM38
Plasmid#162808PurposeSTELLA plasmid for amber suppression of N-terminally labelled SMIM38DepositorInsertUb-(N-TAG)SMIM38
UsePiggybacTagsExpressionMammalianMutationSMIM38 (Met1, Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4x7SKPylT_EF1_Ub-*PIGBOS
Plasmid#162809PurposeSTELLA plasmid for amber suppression of N-terminally labelled PIGBOSDepositorInsertUb-(N-TAG)PIGBOS
UsePiggybacTagsExpressionMammalianMutationPIGBOS (Met1, Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2373
Plasmid#160079PurposeZ. mobilis CRISPRi, promoter F, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2371
Plasmid#160078PurposeZ. mobilis CRISPRi, promoter E, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2369
Plasmid#160077PurposeZ. mobilis CRISPRi, promoter D, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2093
Plasmid#160074PurposeZ. mobilis CRISPRi, promoter B, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-CylI
Plasmid#48200PurposeExpresses CylI (type III PKS) in E. coliDepositorInsertCylI
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-29b-CylB
Plasmid#48199PurposeExpresses CylB (acyl carrier protein) in E. coliDepositorInsertCylB
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
NPY-mCherry
Plasmid#67156PurposeExpresses NPY-mCherryDepositorInsertNPYmCherry (NPY Human)
UseTagsmCherryExpressionMammalianMutationPromotercmvAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJS124
Plasmid#207593PurposeGFP-BIB H20A expressionDepositorInsertGFP-BIB H20A
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-mNeonGreen-CAMSAP3
Plasmid#191322PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flowDepositorInsertmNeonGreen-CAMSAP3 (Camsap3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-mKelly2
Plasmid#119783PurposeExpresses mKelly2 tagged N-terminally with an MHHHHHHGSG tagDepositorInsertmKelly2
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-S9
Plasmid#132948PurposeExpresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coliDepositorInsert30S ribosomal subunit protein S9 (rpsI E.coli)
UseTagsN-terminal 6xHis-tagExpressionBacterialMutationE52CPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD
Plasmid#162804PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI_IRES_Blasticidin
UsePiggybacTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-mKelly1
Plasmid#119782PurposeExpresses mKelly1 tagged N-terminally with an MHHHHHHGSG tagDepositorInsertmKelly1
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2
Plasmid#191338PurposeExpresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice culturesDepositorInsertTUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-Halo-CAMSAP3
Plasmid#191329PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flowDepositorInsertHalo-CAMSAP3 (Camsap3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_NoBody*_Intein_DD
Plasmid#162813PurposeSTELLA plasmid for amber suppression of C-terminally labelled NoBodyDepositorInsertNoBody(C-TAG)-Intein-DD
UsePiggybacTagsExpressionMammalianMutationNoBody(Opal69,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only