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PurposeExpresses NPY-mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepeGFP(N1)
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Backbone manufacturerclontech
- Backbone size w/o insert (bp) 4733
- Total vector size (bp) 5007
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNPYmCherry
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SpeciesH. sapiens (human)
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Insert Size (bp)1040
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GenBank IDNM_000905.3
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Entrez GeneNPY (a.k.a. PYY4)
- Promoter cmv
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site BsrGI (unknown if destroyed)
- 5′ sequencing primer cmv sequence (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NPY-mCherry was a gift from Wolfhard Almers (Addgene plasmid # 67156 ; http://n2t.net/addgene:67156 ; RRID:Addgene_67156) -
For your References section:
Syntaxin clusters assemble reversibly at sites of secretory granules in live cells. Barg S, Knowles MK, Chen X, Midorikawa M, Almers W. Proc Natl Acad Sci U S A. 2010 Nov 30;107(48):20804-9. doi: 10.1073/pnas.1014823107. Epub 2010 Nov 12. 10.1073/pnas.1014823107 PubMed 21076041