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Addgene

NPY-mCherry
(Plasmid #67156)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67156 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    peGFP(N1)
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 4733
  • Total vector size (bp) 5007
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NPYmCherry
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1040
  • GenBank ID
    NM_000905.3
  • Entrez Gene
    NPY (a.k.a. PYY4)
  • Promoter cmv
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site BsrGI (unknown if destroyed)
  • 5′ sequencing primer cmv sequence
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NPY-mCherry was a gift from Wolfhard Almers (Addgene plasmid # 67156 ; http://n2t.net/addgene:67156 ; RRID:Addgene_67156)
  • For your References section:

    Syntaxin clusters assemble reversibly at sites of secretory granules in live cells. Barg S, Knowles MK, Chen X, Midorikawa M, Almers W. Proc Natl Acad Sci U S A. 2010 Nov 30;107(48):20804-9. doi: 10.1073/pnas.1014823107. Epub 2010 Nov 12. 10.1073/pnas.1014823107 PubMed 21076041