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pcDNA3 AIF-mScarlet

Plasmid

#129576

Purpose

Expression of mScarlet fused AIF

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

AIF (AIFM1 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherryC1 VAP-B KD/MD

Plasmid

#226410

Purpose

Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

VAP-B (VAPB Human)

Use

Tags

HA, T7-Xpress tag, and mCherry

Expression

Mammalian

Mutation

K87D/M89D mutant, unable to bind FFAT motifs

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG:myr-mRFP1

Plasmid

#32604

Depositor

Anna-Katerina Hadjantonakis

Article

Nowotschin et al Genesis. 2009 May;47(5):330-6.

Insert

myr-mRFP1

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

pCAGGs

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only
pBAD33(A-N)

Plasmid

#187084

Purpose

Medium expression of Complex I with subunit N-HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Use

Tags

HA tag nuoN

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD33(EFG)

Plasmid

#187087

Purpose

Medium expression of subunits EFG

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoEFG

Use

Tags

HA tag nuoG

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-H*HA-N)

Plasmid

#187086

Purpose

Medium expression of Complex I with subunits N and H -HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Use

Tags

HA tags nuoN, nuoH

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Lyn11-FRB

Plasmid

#155228

Purpose

membrane anchor for FKBP-FRB system

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Membrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-CaaX

Plasmid

#155233

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

YFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-CaaX

Plasmid

#155232

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

CFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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C1-iRFP-iLid-CaaX

Plasmid

#155229

Purpose

membrane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

iRFP C-term tagged with the ilID motif and the CaaX sequence derived from Kras4b. Membrane control

Use

Tags

iRFP-iLid

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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YCe4520 HC_Kan_conn_BsaI-1_p1

Plasmid

#100668

Purpose

EMMA toolkit Assembly connector for second step assembly

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector for second step assembly

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2725 HC_Kan_conn_BsaI-2_p24

Plasmid

#100669

Purpose

EMMA toolkit Assembly connector for second step assembly

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector for second step assembly

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe4522 HC_Kan_conn_BsaI-3_p1

Plasmid

#100670

Purpose

EMMA toolkit Assembly connector for second step assembly

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector for second step assembly

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2730 HC_Kan_conn_BsaI-4_p25

Plasmid

#100671

Purpose

EMMA toolkit Assembly connector for second step assembly

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector for second step assembly

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab2WT

Plasmid

#46779

Purpose

expression of WT Rab2a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB2 (RAB2A Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
mEos3.2trCD45

Plasmid

#203753

Purpose

Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trCD45 (PTPRC Human)

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab2 S20N

Plasmid

#46780

Purpose

expression of S20N Rab2a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB2 (RAB2A Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S20N dominant negative

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
trPAGmEos3.2

Plasmid

#203752

Purpose

Encodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trPAG (PAG1 Human)

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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LBR EGFP

Plasmid

#128150

Purpose

Expression of lamin B receptor

Depositor

Jennifer Lippincott-Schwartz

Article

Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

Insert

lamin B receptor (LBR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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trLATmEos3.2

Plasmid

#203745

Purpose

Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLAT (LAT Human)

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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