mEos3.2trCD45
(Plasmid
#203753)
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PurposeEncodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203753 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemEos3.2-C1
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Backbone manufacturerAddgene; Michael Davidson's lab
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametrCD45
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SpeciesH. sapiens (human)
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Entrez GenePTPRC (a.k.a. B220, CD45, CD45R, GP180, IMD105, L-CA, LCA, LY5, T200)
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Tag
/ Fusion Protein
- mEos3.2 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byInsert DNA by the Sarah Veatch lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEos3.2trCD45 was a gift from Sarah Veatch (Addgene plasmid # 203753 ; http://n2t.net/addgene:203753 ; RRID:Addgene_203753) -
For your References section:
Membrane phase separation drives responsive assembly of receptor signaling domains. Shelby SA, Castello-Serrano I, Wisser KC, Levental I, Veatch SL. Nat Chem Biol. 2023 Jun;19(6):750-758. doi: 10.1038/s41589-023-01268-8. Epub 2023 Mar 30. 10.1038/s41589-023-01268-8 PubMed 36997644