YCe2725 HC_Kan_conn_BsaI-2_p24
(Plasmid
#100669)
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PurposeEMMA toolkit Assembly connector for second step assembly
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSMART HC_Kan
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEMMA toolkit Assembly connector for second step assembly
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DNA element with no function and internal BsaI site generating a ATCC overhang upon cleavage. It is used in the first step BsmBI-based Golden Gate along with HC_Kan_conn_BsaI-1_p1 to flank the vector with BsaI stes. Vectors built in the first BsmBI-based Golden Gate reaction will be flanked by BsaI sites that are used in a second step assembly using as entry vector HC_RFP-4 (the part-entry vector for position 4). See manuscript for futher information
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YCe2725 HC_Kan_conn_BsaI-2_p24 was a gift from Yizhi Cai (Addgene plasmid # 100669 ; http://n2t.net/addgene:100669 ; RRID:Addgene_100669) -
For your References section:
EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y. ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. 10.1021/acssynbio.7b00016 PubMed 28418644