-
Plasmid#177866PurposeCloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassetteDepositorInsertFLuc-based EXSISERS
UseCRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…TagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINT-PuroDDEYFP
Plasmid#31442DepositorInsertenhanced yellow fluorescent protein
UsePhic31/attbTagsFKBP L106PExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTS2353_Tier3(PB)-YPet_PuroR
Plasmid#169652PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-shMePCE_shLARP7
Plasmid#113538PurposeRetroviral vector designed to knock down MePCE and LARP7 simultaneously.DepositorInsertshMePCE and shLARP7 (MEPCE Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-IRESMuro
Plasmid#82336PurposeBase vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertIRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGCGFP-puro
Plasmid#31266DepositorInsertGCGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pISH-Neurod1
Plasmid#74396PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Neurod1. Not for gene expression.DepositorInsertneurogenic differentiation 1 (Neurod1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-Haunt E1-PuroT2AmCherry-4A KI
Plasmid#92164PurposeFor lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockinDepositorInsertHaunt P-Inr2-cDNA KI homology arms
UseOtherTagsExpressionMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBC2-PuroIII
Plasmid#153420PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Puro_LPAR_AKAR-WT
Plasmid#125206PurposeSB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporterDepositorInsertLPAR_AKAR-WT
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor
Plasmid#178087PurposeHDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertmScarlet-I mTOR signaling indicator (TOSI)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Ptgs2_Balbc
Plasmid#170301PurposeA knock-out vector for the mouse Ptgs2 in Balb/cDepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mtagtdTom_IRESMuro
Plasmid#82355PurposeVector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertmtagTandemTomato-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Neurog1
Plasmid#74395PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Neurog1. Not for gene expression.DepositorInsertneurogenin 1 (Neurog1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro KR ATG3
Plasmid#26927DepositorInsertautophagy-related 3 (yeast) (Atg3 Mouse)
UseRetroviralTagsHis and V5ExpressionMammalianMutationChanged Lysine 243 to Arginine.PromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.puro_shHuR 3UTR
Plasmid#110414PurposeTRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selectionDepositorInsertELAVL1 (HuR)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA PolIII)Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-LDR
Plasmid#173857PurposeEncoding a part of the rapamycin-induced dimerization system.DepositorInserta Lyn11-targeted FRB (LDR)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
33-G-X1-pEF1-puro
Plasmid#111604PurposeExpress human 33-G-X1 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
UseTagsFlag tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533AK570A
Plasmid#14104DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to Ala, Lys570 to AlaninePromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-HCF-1-D
Plasmid#36997DepositorInsertHCF-1 shRNA (HCFC1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro-Willin
Plasmid#40884DepositorInsertWillin (FRMD6 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Neuroserpin
Plasmid#58259PurposeRetroviral vector for expressing Neuroserpin in mammalian cellsDepositorInsertNeuroserpin (SERPINI1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-HCF-1-B
Plasmid#36996DepositorInsertHCF-1 shRNA (HCFC1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Gnaq
Plasmid#170302PurposeA knock-out vector for the mouse GnaqDepositorInsertA gRNA targeting the mouse Gnaq gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
ST-SBP-mCherry_puromycin
Plasmid#65271PurposeST reporter only ( no hook) (RUSH system)DepositorInserttruncated sialyltransferase (ST6GAL1 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRRLSIN.cPPT.PGK.NeuroD2.WPRE
Plasmid#122054PurposeLentiviral expression of NeuroD2DepositorInsertNeuroD2 (Neurod2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_TLOC1 (220aa)
Plasmid#45273DepositorInsertTLOC1 (SEC62 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABEPuro-rnSOX9
Plasmid#63009PurposeExpresses rat SOX9DepositorInsertSOX9 (Sox9 Rat)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-MaSgRNA_PuroR
Plasmid#84780PurposeExpresses major satellite-specific sgRNA.DepositorInsertmajor satellite-specific sgRNA
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceJuly 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-H-RAS_V12
Plasmid#39526DepositorInsertHRAS V12 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationV12 = G12VPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSc1-puro
Plasmid#80438PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycinDepositorInsertssp Cas9 gRNA
eGFP
Puromycin resistance
UseCRISPRTagsExpressionMammalianMutationPromoterCMV, U6, and hPGKAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSUPERIOR.puro-shREV3-4
Plasmid#38029DepositorInsertshREV3-4 (REV3L Human, Mouse, human, Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQCXIpuro-ITGA5
Plasmid#134764Purposea retroviral vector to express integrin alpha5DepositorInsertITGA5 (ITGA5 )
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINS-Puro
Plasmid#31389DepositorInsertpac
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
6691 Bicistronic_ires_puro
Plasmid#64335PurposeThis a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance geneDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pScalps_puro_mIL-2Rα RK
Plasmid#59920PurposeExpresses mouse interleukin-2 receptor alpha chain mutatedDepositorInsertinterleukin 2 receptor alpha (Il2ra Mouse)
UseLentiviralTagsExpressionMutationchanged sequence of the intracellular (C-terminal…PromoterAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM_Puro_shRNA n.2_mTet3
Plasmid#79625PurposeExpression of shRNA for mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseLentiviralTagsnoneExpressionMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGM_Puro_shRNA n.1_mTet3
Plasmid#79624PurposeExpression of shRNA for mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseLentiviralTagsnoneExpressionMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-MiSgRNA_PuroR
Plasmid#84781PurposeExpresses minor satellite-specific sgRNA.DepositorInsertminor satellite-specific sgRNA
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVKG1-Puro
Plasmid#108670PurposePuromycin resistant cassette for knock-in by VIKING methodDepositorInsertPURO
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLIC-puro
Plasmid#45197Purposea ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUPERIOR.puro-shREV3-6
Plasmid#38031DepositorInsertshREV3-6 (REV3L Human, human, Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSUPERIOR.puro-shREV3-5
Plasmid#38030DepositorInsertshREV3-5 (REV3L Human, human, Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-shMePCE
Plasmid#113536PurposeRetroviral vector designed to knock down MePCE.DepositorInsertshMePCE (MEPCE Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2Q545AK570A
Plasmid#14105DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Gln545 to Ala and Lys570 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGalT_EGFP-IRES_puro2b
Plasmid#31804DepositorInsertpGALT
UseTagsExpressionMammalianMutation*see commentsPromoterAvailable sinceOct. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-CRAF_S257W
Plasmid#51126Purposemammalian expression of wt CRAF S257WDepositorInsertCRAF (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutationS257WPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K570A
Plasmid#14102DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys570 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ARAF_S214T
Plasmid#51079Purposemammalian expression of ARAF S214TDepositorInsertARAF (ARAF Human)
UseRetroviralTagsExpressionMammalianMutationS214TPromoterAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ARAF_S214F
Plasmid#51078Purposemammalian expression of ARAF S214FDepositorInsertARAF (ARAF Human)
UseRetroviralTagsExpressionMammalianMutationS214FPromoterAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only