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Plasmid#192525PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertIAA-leu-resistant-like5 (ILL5 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterILL5Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPPC017.109
Plasmid#171145PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC020.306
Plasmid#171148PurposeExpression of J3-BBa_J23117-mRFP and J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLLX8
Plasmid#79838PurposeContains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vectorDepositorTypeEmpty backboneUseContains yeast counter selection gene, cyh2 and b…TagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
PA1375_pET21a(+)C-tag
Plasmid#14580DepositorInsertD-Erythronate-4-phosphate Dehydrogenase (pdxB Pseudomonas aeruginosa)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pS44i8GH-2
Plasmid#207526PurposeConditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; SmR/SpRDepositorInsertoriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPPC031
Plasmid#171152PurposepGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassetteDepositorInsertJ3-BBa_J23117-mRFP
UseTagsExpressionBacterialMutationPromoterJ3-BBa_J23117Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC034
Plasmid#171153PurposepGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassetteDepositorInsertJ3(106)- BBa_J23111-sfGFP
UseTagsExpressionBacterialMutationPromoterJ3(106)-BBa_J23111Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC005
Plasmid#171140PurposeFor integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertXylS-Pm
UseCRISPRTagsExpressionBacterialMutationPromoterPmAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC018.AAV
Plasmid#171146PurposeExpression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmidDepositorInsertshAAVS1 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC016.109
Plasmid#171144PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC019.AAV
Plasmid#171147PurposeExpression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmidDepositorInsertshAAVS1 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-tglA(C51)HIB
Plasmid#134675PurposeExpresses His6-TglA(C51), TglH, TglI and TglB in E. ColiDepositorInsertsTglA(C51)
TglH
TglI
TglB
UseTagsHis6ExpressionBacterialMutationinsertion of cysteine 51PromoterT7Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)
Plasmid#108239PurposeExpresses PdR-PCNA2(L171C) fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C) fusion protein
UseTagsHis6 tagExpressionBacterialMutationPCNA2,L171CPromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPD3cC
Plasmid#86972PurposeExpresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …PromoterT7 promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1G108CP20X
Plasmid#85096PurposeExpresses PCNA1 variant (G108C) fused to putidaredoxin in E. coliDepositorInsertThe G108C variant of PCNA1 fused to putidaredoxin
UseTagsHis6 tagExpressionBacterialMutationG108C in PCNA1PromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPApqsE
Plasmid#97019PurposeExpressing Pseudomonas aeruginosa pqsE proteinDepositorInsertQuinolone signal response protein
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPAFabH
Plasmid#97018PurposeExpressing Pseudomonas aeruginosa FabH proteinDepositorInsert3-Oxoacyl-[acyl-carrier-protein] synthase III
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA5006
Plasmid#97016PurposeExpressing Pseudomonas aeruginosa orf PA5006 proteinDepositorInsertP aeruginosa orf PA5006
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA0856
Plasmid#97014PurposeExpressing Pseudomonas aeruginosa orf PA0856 proteinDepositorInsertP aeruginosa orf PA0856
UseTagsN-ter TEV cleavable 6HIsExpressionMutationN-terminal deletion (AA 1-34)PromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA4631
Plasmid#97017PurposeExpressing Pseudomonas aeruginosa orf PA4631 proteinDepositorInsertNucleoside-diphosphate-sugar epimerase
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPApqsL
Plasmid#97020PurposeExpressing Pseudomonas aeruginosa pqsL proteinDepositorInsertPutative FAD-dependent monooxygenase
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA4511
Plasmid#97015PurposeExpressing Pseudomonas aeruginosa orf PA4511 proteinDepositorInsertP. aeruginosa orf PA4511
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pS648∙CsR
Plasmid#197860PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS548∙CsR
Plasmid#197859PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Tc resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2-6xHis
Plasmid#218885PurposeBacterial expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseTags6xHisExpressionBacterialMutationS99A F102Y F104Y L178SPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-iGABASnFR2-WPRE-bGH-polyA
Plasmid#218868PurposeMammalian expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA
Plasmid#218869PurposeMammalian expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseTagsExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pS44i8GM
Plasmid#207527PurposeConditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpRDepositorInsertoriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR1G108CP20X
Plasmid#85086PurposeExpresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coliDepositorInsertThe G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
UseTagsHis6 tagExpressionBacterialMutationG108C in PCNA1PromoterT7 promoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2n-6xHis
Plasmid#218886PurposeBacterial expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseTags6xHisExpressionBacterialMutationS99A F104H R168PPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
UseTags6xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
UseTags8xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)
Plasmid#110560PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insertDepositorInsertstRBS-lacZ
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVRc20_992
Plasmid#49739DepositorInsertAS20_992
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pS848∙CsR
Plasmid#199240PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-BenR
Plasmid#114597Purpose"Strong constitutive expression of transcription factor, BenR, for cell-free expression"DepositorInsertBenR
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterOR2-OR1-PrAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLLX13
Plasmid#79825PurposeYeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vectorDepositorTypeEmpty backboneUseYeast and bacteria shuttle vector, contains origi…TagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVRa16_3622
Plasmid#49670DepositorInsertECF16_3622
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)
Plasmid#110561PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insertDepositorInsertstRBS-aacC1
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1-Pct5.1-crRNA(dadR)
Plasmid#191634PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)DepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMA3383
Plasmid#46875PurposeA retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3DepositorInsertsoluble guanylate cyclase 1beta 3 (Gucy1b3 Rat)
UseRetroviralTagsExpressionMutationPromoterLTRAvailable sinceAug. 13, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPPC027.306
Plasmid#171149PurposeBiopterin production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)
Plasmid#108195PurposeExpresses PdR-PCNA3(R112C/T180C) fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C) fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVRc35_3582
Plasmid#49750DepositorInsertAS35_3582
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc16_3622
Plasmid#49738DepositorInsertAS16_3622
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa16_973
Plasmid#49669DepositorInsertECF16_973
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
PA1024_pET21a(+)C-tag
Plasmid#14579DepositorInsert2-nitropropane dioxygenase (PA1024 Pseudomonas aeruginosa)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-R115A/R119A
Plasmid#80696PurposeExpresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-R115A/R119A
UseAAVTagsExpressionMammalianMutationArg115 and Arg119 both mutated to AlaPromoterCBAAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA
Plasmid#218870PurposeMammalian expression of improved GABA sensor control (no change in fluorescence)DepositorInsertiGABASnFR2(no bind)
UseTagsExpressionMammalianMutationS99A F102G R168PPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only