-
Plasmid#102565Purposebacterial expression of AsCpf1-2NLSDepositorInsertAsCpf1
UseTags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR
Plasmid#81251PurposeLentiviral construct for building stable cell lines expressing Cas9 via puromycin selectionDepositorInsertCas9-3xNLS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-2NLSCas9
Plasmid#53064PurposeExpression of rice codon-optimized Cas9 in plant cellsDepositorInsertCas9
UsePlant expressionTagsExpressionMutationRice codon optimizedPromoter2x35SAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMVMagFRET-1-NLS
Plasmid#50748PurposeExpresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.DepositorInsertgene encoding for MagFRET-1 with NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAi14-GFPNLS-MC
Plasmid#87114Purposeminicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITIDepositorInsertGFPNLSPpA
UseCRISPRTagsExpressionMutationPromoternEFAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
M_NLS 41kDa
Plasmid#201341PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A5-EGFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP
Plasmid#162233PurposeLentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cellsDepositorInsertanti-GFP nanobody lag16lag2 and IFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR'CMVGFP_NLS_hRIF1(1-1308)
Plasmid#23134DepositorInsertRIF1 deletion construct (RIF1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaNLS_P2A_Hygro_Barcode
Plasmid#120506PurposeBarcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC H84NLS (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of nuclear localization signal sequencePromoterEF1aAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_2xNLS_LbCpf1_protein_expression
Plasmid#114366PurposeProtein expression plasmid for 2xNLS LbCpf1 in pET-21a backboneDepositorInsert2xNLS_LbCpf1
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS
Plasmid#79372PurposeRecruits p65-hsf1 to a compatible Cas9 protein for transcriptional activationDepositorInsertGCN4-sfGFP-p65-hsf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
p16 nlsGPS1-NR
Plasmid#101082PurposeGA biosensor expression plasmidDepositorInsertnlsGPS1-NR
UseTagscMycExpressionPlantMutationAtGAI E51K E54R, AtGID1C S114A F115APromoterp16 (AT3G60245)Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
1xNLS-pMJ915v2
Plasmid#88915PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_FnCpf1_2xNLS_protein_expression
Plasmid#124548PurposeProtein expression plasmid for 2xNLS FnCpf1 in pET-21a backboneDepositorInsertFnCpf1_2xNLS
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMVMagFRET-6-NLS
Plasmid#50757PurposeExpresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1DepositorInsertgene encoding for MagFRET-6 with NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS-nlstdTomato
Plasmid#132985Purposefull length of nuclear localized tdTomato in pCS expression vectorDepositorInserttdTomato
UseTagsNLS and mycExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWPT-nlsLacZ
Plasmid#12261DepositorInsertLacZ
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS-HA-B3
Plasmid#51270PurposeExpression of the site-specific recombinase B3 (mouse codon-optimized)DepositorInsertNLS-HA-B3
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E-2a-6MTnlstdTomato
Plasmid#67699Purpose3' entry clone with 6xmyc nuclear localized tdTomatoDepositorInserttdTomato
UseTags2A-6xMyc-NLSExpressionMutationPromoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
R26/P-nlsrtTA
Plasmid#46183DepositorInsertsnlsrtTA
pgk-puro
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
M_NLS 67kDa
Plasmid#201351PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A5-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
L_NLS 67kDa
Plasmid#201361PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A4-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
H_NLS 67kDa
Plasmid#201372PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
LN-entry+NLS
Plasmid#128011PurposeVector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag-SV40NLS
UseLamdan entry vector with nls to generate tetherin…TagsLN-3xFlag-SV40NLSExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-mRFP-V1
Plasmid#124266PurposeNuclear localised mRFP reporter construct with one half of split VenusDepositorInsertmRFP
UseTagsNLS and Venus1ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-GAI(1-92)
Plasmid#37317DepositorInsertGAI (GAI Mustard Weed)
UseTagsNLSExpressionMammalianMutationcontains aa 1-92PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3E-CMVmin:nlsGFP pA
Plasmid#80821PurposeCMVmin-promoted nlsGFP with pADepositorInsertp3E-CMV-nlsGFP pA
UseTagsExpressionMammalianMutationPromoterCMVminAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNLS-LSSmKate1-N1
Plasmid#31870DepositorInsertNLS
UseTagsExpressionMammalianMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterCMVAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_2xNLS_FnCpf1_MBP_protein_expression
Plasmid#120801PurposeProtein expression plasmid for 2xNLS FnCpf1 in pET-21a backboneDepositorInsert2xNLS_FnCpf1
UseCRISPRTagsMBP and NLSExpressionBacterialMutationPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40
Plasmid#100030PurposeAAV expression of ArcLightDDepositorInsertArcLightD
UseAAVTags2A-mCherryExpressionMammalianMutationPromoterCBAAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ oDam_f_GFPoNLS
Plasmid#85819PurposeiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertoDam_f_GFPoNLS
UseTagsoNLS (optimized nuclear localization signal) C te…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-BE3-2NLS
Plasmid#119031PurposeStably express BE3 in mammalian cellsDepositorInsertBE3
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNLS-LSSmKate2-N1
Plasmid#31871DepositorInsertNLS
UseTagsExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…PromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT2iC6Dronpa2nlsER4(#636)
Plasmid#184067Purposecyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesisDepositorInsertDronpa2-2nls-ERT2
UseSynthetic BiologyTagsExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-mRFP-V2
Plasmid#124267PurposeNuclear localised mRFP reporter construct with one half of split VenusDepositorInsertmRFP
UseTagsNLS and Venus2ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-6MTnlstdTomato2A
Plasmid#67685PurposeMiddle entry nuclear localized tdTomato reporter with 6xMT and 2ADepositorInserttdTomato
UseTags2A and 6xMyc-NLSExpressionMutationPromoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-BE3-3NLS
Plasmid#119032PurposeStably express BE3 in mammalian cellsDepositorInsertBE3
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P4:gs:nls:FKBP
Plasmid#135984PurposeExpresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cellsDepositorInsertP4 coiled-coil fused to the FKBP domain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:FRB:nls:VPR
Plasmid#135987PurposeExpresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cellsDepositorInsertFRB domain fused to the VPR activation domain
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LI B-C N-NLS KOZ c2
Plasmid#54443DepositorInsertNuclear localization signal (NLS) of SV40 large T antigen
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E-nlsGFP pA
Plasmid#80814PurposenlsGFP with pADepositorInsertnlsGFPpA 3'E
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_3xNLS
Plasmid#65673PurposeExpresses NLS-CNL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_3xNLS
Plasmid#65672PurposeExpresses NLS-YNL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_3xNLS
Plasmid#65674PurposeExpresses NLS-ONL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
p16 nlsGPS2
Plasmid#228093PurposeExpression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)DepositorInsertnlsGPS2
UseTags6xHis-T7 XpressTag and cMycExpressionPlantMutationPromoterp16Available sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABE8e–superNLS
Plasmid#232429PurposeExpression plasmid for the ABE8e base editor with superNLSDepositorInsertABE8e–superNLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Cas9–superNLS
Plasmid#232430PurposeExpression plasmid for the SpCas9 nuclease with superNLSDepositorInsertCas9–superNLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR
Plasmid#203964PurposeDox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)DepositorInsertSOX9-noHMG (Sox9 Mouse)
UseLentiviralTagsmycExpressionMutationthe HMG domain is truncatedPromoterTRE3GAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
L_NLS 80kDa
Plasmid#201387PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A4-EGFP-8PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
M_NLS 80kDa
Plasmid#201386PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A5-EGFP-8PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only