...region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) an RFP protein that binds to the 3’ UTR ...UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains the tethering... to appear yellow. The act of translation pushes NLS-PCP-GFP off of the mRNA, leaving only a red FP bound...Because it contains a nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind cognate...allow continued tracking of a given mRNA because NLS-PCP-GFP is removed by the first ribosome that translates...

...pAAV-FLEX-tdTomato pAAV-GFAP104-mCherry pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn ...pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry at Addgene. Viral...

...62933 pET-Cas9-NLS-6xHis 6xHis (C terminal); NLS (C terminal) 62934 pET-NLS-Cas9-6xHis 6xHis...(N terminal); 2xNLS (C terminal) 47327 pET-28b-Cas9-His 6xHis (C terminal); NLS (C terminal) ...6xHis (C terminal); NLS (N terminal)   Easi-CRISPR efficiency and delivery methods Using RNP delivery...

...AAV9 Biosensor Looger New serotype pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics Adesnik ...7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2  AAV PHP.eB Controls Gradinaru New serotype...

...nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters the nucleus where...Mxi1 can repress gene expression. In the dark, the NLS is inaccessible and the complex remains in the cytoplasm...

...and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein...

...activation. Multiple nuclear localization signals (NLS) increase protein import into the nucleus. In developing...

...AAV5 pAAV-Ef1a-fDIO mCherry 99130 AAVrg pAAV-mDlx-NLS-mRuby2 112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a ...

...promoter directs ubiquitous expression. pAAV-mDlx-NLS-mRuby2 (99130-AAVrg): The Dlx element directs expression...

...AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d...

...enough to target larger areas, typically a few hundred nl. Alternatively, for larger brain areas, repeated ...

...EGFP/NLS Ple12 AVP pEMS1241 EGFP/NLS Ple12 AVP pEMS1482 intron-lacZ/NLS Ple13 AVP pEMS1242 EGFP/NLS Ple15...intron-lacZ/NLS Ple36 CRH pEMS1153 EGFP/NLS Ple37 CRH pEMS1154 EGFP/NLS Ple38 CRH pEMS1155 EGFP/NLS Ple39 CRH...cre/NLS Ple50 DBH pEMS1361 EGFP/NLS Ple51 DBH pEMS1113 EGFP/cre/NLS Ple51 DBH pEMS1362 EGFP/NLS Ple52 DCX...EGFP/NLS Ple53 DCX pEMS1199 EGFP/NLS Ple53 DCX pEMS1524 intron-lacZ/NLS Ple54 DCX pEMS1200 EGFP/NLS Ple55...intron-lacZ/NLS Ple56 DDC pEMS1125 EGFP/NLS Ple57 DDC pEMS1126 EGFP/NLS Ple58 DDC pEMS1127 EGFP/NLS Ple58 DDC...EGFP/NLS Ple63 DRD1 pEMS1168 EGFP/NLS Ple64 FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS Ple66...EGFP/NLS Ple81 GAL pEMS1177 EGFP/NLS Ple84 GAL pEMS1180 EGFP/NLS Ple85 GCHFR pEMS1122 EGFP/cre/NLS Ple85...

...j.cell.2016.11.017 Shin J, Jiang F, Liu J-J, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn...

...containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP 46911 pHR-SFFV-dCas9-BFP-KRAB Human expression...containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain 46912 pMSCV-LTR-dCas9-VP64...containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP 51023 pSLQ1658-dCas9-EGFP Human ...expression vector containing dCas9 that is fused to 2x NLS and EGFP for CRISPR imaging 51024 pSLQ1651-sgTelomere...containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP 46914 pU6-sgGFP-NT1...CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter 46921 pTDH3-dCas9-Mxi1...

...Nogueira ML, Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti ...

...8, 9, rg*, PHP.eB Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx NLS-mRuby2 Constitutive 1, 2, 8, 9, rg*, PHP....Constitutive 2, 5, rg*, PHP.eB Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH...pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre dependent 1, 2, 5, 8, 9, rg* Harvey...Constitutive 1, 8 Deisseroth 135630 pAAV-S5E2-dTom-nlsdTom E2 regulatory dTomato Constitutive 1, 9, PHP.eB...oScarlet Constitutive 8 Deisseroth 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive...

... 37354 pQC NLS mCherry IX Nucleus NLS mCherry Connie Cepko 37341 pQC NLS YFP IX Nucleus NLS YFP Connie...pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois St-Pierre 14893 pEBFP2-Nuc Nucleus NLS EBFP2 ...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...98875 3xnls-mNeonGreen Nucleus 3xNLS mNeonGreen Dorus Gadella 98817 3xnls-mTurquoise2 Nucleus 3xNLS mTurquoise2...mTurquoise2 Dorus Gadella 98816 3xnls-mScarlet-I Nucleus 3xNLS mScarlet-I Dorus Gadella 98876 4xmts-mNeonGreen...

...Flp-dependent Control Deisseroth 99130 pAAV-mDlx-NLS-mRuby2 Dlx NLS-mRuby2 Control Gradinaru 83900 pAAV-mDlx-...Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells...Recombinases Janelia 51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear...Calcium sensor Ting 51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear...Calcium sensor Fishell 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear...

...region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of... of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains the tethering...are transported back to the nucleus (thanks to a NLS on these proteins). The RFP proteins, however, remain...wild-type Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments facilitates target DNA...fused with FK 506 binding protein 12 (Cas9(C)-FKBP-2xNLS) and the n-terminal cas9 fragment with FKBP rapamycin...rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS dimerization and net influx into the nucleus, where...using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This system provides...

...W, Dion SL, Kutny PM, Zhang Y, Cheng AW, Jillette NL, Malhotra A, Geurts AM, Chen Y-G, Wang H (2015) Efficient...