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Plasmid#112788PurposeDownstream homology arm, SpsC is involved in spore coat polysaccharide synthesisDepositorArticleInsertspsCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-spsCU
Plasmid#112787PurposeUpstream homology arm, SpsC is involved in spore coat polysaccharide synthesisDepositorArticleInsertspsCU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22HT-PP7delFG
Plasmid#31863DepositorInsertPP7delFG Coat Protein
UseTagsHis6-tag and TEV siteExpressionMutationdeleted FG loopPromoterAvailable sinceAug. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMS2-1
Plasmid#220627PurposePlasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - mcp - egfp - BirA*
Plasmid#131134PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-MCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - mCherry - BirA*
Plasmid#131136PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-mCherry-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS2-2
Plasmid#220628PurposePlasmid allows you to place the RNA sequence of interest next to two MS2 coat protein recognition sites.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - cMycBirA*
Plasmid#131133PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-cMycBirA*
UseLentiviralTagsNLS, HA, cMyc (N-terminal of BirA*)ExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEF1_MS2
Plasmid#220625PurposeSource for TEF1 MS2 coat fusion protein fusionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
phage-ubc-nls-ha-tdMCP-gfp
Plasmid#40649DepositorInserttdMCP-gfp
UseLentiviralTagsEGFP, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
phage-ubc-nls-ha-tdPCP-gfp
Plasmid#40650DepositorInserttdPCP-gfp
UseLentiviralTagsEGFP, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSX2719
Plasmid#200763PurposeExpresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with the tissue-specific promoter semo-1 in C. elegans.DepositorInsertMCP-24xGCN4
UseTagsExpressionWormMutationPromotersemo-1Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIVMD217
Plasmid#160939PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mTagBFP2-HA Tag
UseTagsHA tagExpressionYeastMutationPromoterMET25Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry
Plasmid#207668PurposeTo express fluorescently labeled MS2 coat binding protein (MCP)DepositorInsertMCP-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
MS2-p65-HSF1-P2A-mCherry
Plasmid#184440PurposeMS2 coat protein fused with p65 and hsf1DepositorInsertMS2-p65-hsf1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-Tet1-CD
Plasmid#83341PurposeTo introduce MS2 coat protein fused Tet1-CDDepositorInsertMS2-Tet1-CD
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_Delta
Plasmid#175950PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationDelta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDZ274 (pLEU MET25pro MCP-2x-yeGFP)
Plasmid#45929DepositorInsertMCP-2x-yeGFP
UseTags2x-yeGFPExpressionYeastMutationPromoterMET25Available sinceAug. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAP72_MS2
Plasmid#220623PurposeCassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.DepositorTypeEmpty backboneUseShuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSH100 (YCplac33 MET25pro MCP-mCherry)
Plasmid#45930DepositorInsertMCP-mCherry
UseTagsmCherryExpressionYeastMutationPromoterMet25Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMS2CP-myc-T2A-tagRFP
Plasmid#140259PurposeExpresses WT MS2CP in mammalian cellsDepositorInsertCoat protein of bacteriphage MS2
UseSynthetic BiologyTagsmyc-T2A-tagRFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP7CP-myc-T2A-tagRFP
Plasmid#140262PurposeExpresses PP7CP in mammalian cellsDepositorInsertCoat protein of bacteriphage PP7
UseSynthetic BiologyTagsmyc-T2A-tagRFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDZ513 (pHIS MET25pro PP7-PS-yeGFP)
Plasmid#45931DepositorInsertPCP-PS
UseTags2x-yeGFPExpressionYeastMutationPromoterMet25Available sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
CTAG_mScarlet-I
Plasmid#136118PurposeFP for C-term fusion with CDS12DepositorInsertCTAG-mScarletI(noATG)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBT005
Plasmid#177323PurposeMCP-SoxS (R93A) expressionDepositorInsertSoxS
UseTagsMCP (MS2 Coat Protein)ExpressionMutationR93APromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-auxilin(547-910)
Plasmid#62572PurposeCodes for a GST-auxilin fusion protein that is functional as a co-factor in hsc70-dependent clathrin coat disruptionDepositorInsertAuxilin
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_Omicron
Plasmid#180229PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationOmicron (Amino Acid 319-869)PromoterT7Available sinceJan. 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_WT
Plasmid#175044PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationWT (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFTK079
Plasmid#171351PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertToat1 Pc18g03600 terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGMCS-0X-P38-PanBmGFP
Plasmid#27127DepositorInsertPanB
UseTagsLinker (SGSGSG), GFPm2+ExpressionBacterialMutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFTK008
Plasmid#171280PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPoat1 Pc18g03600 promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_pBen-BenR
Plasmid#128127PurposeExpressing BenR transcription factor gene under control of benzoate responsive promoter (pBen) in a feedback loop.DepositorInsertBenR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBenAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_Lambda
Plasmid#175048PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Lambda SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationLambda (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Alpha
Plasmid#175045PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Alpha SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationAlpha (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Beta
Plasmid#175046PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Beta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationBeta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGP-Tn7-Pcym
Plasmid#225280PurposeMini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPcym cumate (p-isopropylbenzoate)-inducible promo…Available sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_HelperΔgIII
Plasmid#190433PurposeM13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.DepositorInsertM13 genes I-V and VII-XI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_B.1.640
Plasmid#180228PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the B.1.640 SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationB.1.640 (Amino Acid 319-869)PromoterT7Available sinceJan. 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_C.1.2
Plasmid#177367PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the C1.2 SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationC1.2 (Amino Acid 319-869)PromoterT7Available sinceJan. 20, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Mu
Plasmid#177366PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Mu SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationMu (Amino Acid 319-869)PromoterT7Available sinceJan. 20, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
CTAG_eGFP
Plasmid#136095PurposeFP for C-term fusion with CDS12DepositorInsertCTAG_eGFP(noATG)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTAG_mTurquoise2
Plasmid#136104PurposeFP for C-term fusion with CDS12DepositorInsertCTAG_mTurquoise2(noATG)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CTAG_mVenus
Plasmid#136106PurposeFP for C-term fusion with CDS12DepositorInsertCTAG_mVenus(noATG)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Kappa
Plasmid#175953PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Kappa SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationKappa (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Eta
Plasmid#175954PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Eta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationEta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Theta
Plasmid#175951PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Theta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationTheta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Zeta
Plasmid#175952PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Zeta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationZeta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Gamma
Plasmid#175047PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Gamma SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationGamma (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
plIVMD118
Plasmid#160938PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mCherry-FLAG tag
UseTagsMYC tagExpressionYeastMutationPromoterMET25Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD115
Plasmid#160937PurposeExcess Capsid Protein for in vivo mRNA displayDepositorInsertMCP-mCherry-MYC tag
UseTagsMYC tagExpressionYeastMutationPromoterMET25Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only