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Addgene

pGMCS-0X-P38-PanBmGFP
(Plasmid #27127)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27127 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGMCS
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PanB
  • Alt name
    Probable 3-methyl-2-oxobutanoate hydroxymethyltransferase
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    842
  • Tags / Fusion Proteins
    • Linker (SGSGSG) (C terminal on insert)
    • mGFP (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway Cloning (att) (unknown if destroyed)
  • 3′ cloning site Gateway Cloning (att) (unknown if destroyed)
  • 5′ sequencing primer M13_puc_Rev
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGMCS-0X-P38-PanBmGFP was a gift from Sabine Ehrt (Addgene plasmid # 27127 ; http://n2t.net/addgene:27127 ; RRID:Addgene_27127)
  • For your References section:

    The Mycobacterium tuberculosis proteasome active site threonine is essential for persistence yet dispensable for replication and resistance to nitric oxide. Gandotra S, Lebron MB, Ehrt S. PLoS Pathog. 2010 Aug 12;6(8):e1001040. doi: 10.1371/journal.ppat.1001040 10.1371/journal.ppat.1001040 PubMed 20711362