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Plasmid#38240DepositorInsert4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationT37A, T46A, S65A, T70A (dominant negative)PromoterTet ONAvailable sinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
FUG+p97W (C2)
Plasmid#74018Purposelentiviral expression of EGFP-SAP97beta fusionDepositorInsertSAP97 beta isoform
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAMPK alpha2 K45R
Plasmid#15992DepositorInsertAMPK alpha2 (Prkaa2 Rat)
UseTagsMycExpressionMammalianMutationK45RPromoterCMVAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA HisB TO rFez1
Plasmid#17385DepositorInsertFEZ1 (Fez1 Rat)
UseTagshis and mycExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.FLEX.iGluSnFR3.v857.GPI
Plasmid#196218PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorInsertpAAV CAG.FLEX iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Vamp3-pHluorin
Plasmid#190152PurposeExpresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosisDepositorInsertVamp3 (Vamp3 Rat)
UseAAVTagssuperecliptic pHluorinExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-lck-jGCaMP8s
Plasmid#176761PurposeAAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoterDepositorHas ServiceAAV1 and AAV9Insertlck-jGCaMP8s
UseAAVTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
YPet-Lysosomes-20
Plasmid#56636PurposeLocalization: Lysosome Membrane, Excitation: 513, Emission: 527DepositorInsertLAMP1
UseTagsYPetExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-BE3
Plasmid#73021PurposeExpresses BE3 in mammalian cellsDepositorInsertBE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-mRFP-FLAG
Plasmid#34611Purpose3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tagDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTags2xFlag and mRFPExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
Cherry-HA-KCC2
Plasmid#104077PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
UseTagsmCherry-HA tagExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-miRFP703
Plasmid#79998PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP703DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,42a]
Plasmid#49333PurposeRepaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…PromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
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CGBE1-NG (pBM1246)
Plasmid#140256PurposeCMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFPDepositorInserteUNG-BE4max(R33A)_NG-ΔUGI
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, NG mutations in SpCas9, D10A in…PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-APOBEC1-YTH
Plasmid#178949PurposeLentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cellsDepositorInsertAPOBEC1-YTH-T2A-GFP
UseLentiviral and Synthetic Biology; InducibleTagsHAExpressionMammalianMutationPromotertight TREAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p130Cas Y15F
Plasmid#186154PurposeFor expressing recombinant p130Cas in insect cells.DepositorInsertBCAR1 (Bcar1 Rat)
UseTagsHis6 (Tev)ExpressionInsectMutation15Y residues mutated to AlaninePromoterpolyhedrinAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-rM3D(Gs)-mCherry
Plasmid#50458PurposeDouble floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorHas ServiceAAV8InsertrM3D-mCherry
UseAAVTagsHA and mCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-APOBEC1-YTHmut
Plasmid#178950PurposeLentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cellsDepositorInsertAPOBEC1-YTHmut-T2A-GFP
UseLentiviral and Synthetic Biology; InducibleTagsHAExpressionMammalianMutationPromotertight TREAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1-SpRY (pBM1571)
Plasmid#170105PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFPDepositorInsertrAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationR33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…PromoterCMVAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only