Showing: 161 - 180 of 8203 results
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Plasmid#70088PurposeEncode YTHDF3 isoform a with N-terminal GST tag for E. coli expressionDepositorInsertYTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pLX302_FOXA1-V5
Plasmid#70090PurposeExpression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vectorDepositorInsertFOXA1 (FOXA1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-hCAS-pA
Plasmid#48625PurposeExpresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized Cas9
UseCRISPRTagsFLAG, NLS, and polyAExpressionMammalianMutationPromoterCAG promoterAvailabilityAcademic Institutions and Nonprofits only -
DS-SPcas
Plasmid#48645PurposeBacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
tracrRNA precursor
UseCRISPRTagsExpressionMutationPromoterproC and tracrRNA promoterAvailabilityAcademic Institutions and Nonprofits only -
M-ST1cas
Plasmid#48669PurposeMammalian S. thermophilus #1 Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
M-NMcas
Plasmid#48670PurposeMammalian N. meningitidis Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
M-ST1-sgRNA
Plasmid#48672PurposeMammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTGDepositorInsertsgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
UseCRISPRTagsExpressionMutationPromoterhUAvailabilityAcademic Institutions and Nonprofits only -
M-NM-sgRNA
Plasmid#48673PurposeMammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTGDepositorInsertsgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
UseCRISPRTagsExpressionMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
M-ST1n-VP64
Plasmid#48675PurposeMammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimizedDepositorInsertCas9-VP64, nuclease-null
UseCRISPRTagsNLS and VP64ExpressionMutationnuclease-null (D9A D598A H599A N622A)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
M-NMn-VP64
Plasmid#48676PurposeMammalian NM-VP64 nuclease-null Cas9 activator expression, human optimizedDepositorInsertCas9-VP64, nuclease-null
UseCRISPRTagsNLS and VP64ExpressionMutationnuclease-nullPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Oct4-IRES-eGFP-PGK-Neo
Plasmid#48681PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorInsertOct4 (Pou5f1 Mouse)
UseCRISPRTagsGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUltra-Chili
Plasmid#48687Purpose3rd generation Lentiviral vector with bi-cistronic expression of dtomato and the gene of interest.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUltra-Chili-Luc
Plasmid#48688Purpose3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferaseDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFMRPiso1
Plasmid#48690PurposeExpresses hFMRP iso 1DepositorInsertFlagHA FMR1 Iso 1 (FMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFXR1
Plasmid#48694PurposeExpresses hFXR1DepositorInsertFlagHA FXR1 (isoform b) (FXR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696Purposeallows co-translational incorporation of a fluorescent AA directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-14-3-3 ϵ
Plasmid#48797Purposeexpresses human myc-14-3-3ϵ in mammalian cellsDepositorInsertYWHAE 14-3-3 ϵ (YWHAE Human)
UseTagshaemagglutininExpressionMammalianMutationPromotercmvAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-14-3-3ζ
Plasmid#48798Purposeexpresses human myc-14-3-3ζ in mammalian cellsDepositorInsertYWHAZ (YWHAZ Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB) (PX460)
Plasmid#48873PurposeCas9n (D10A nickase mutant) from S. pyogenesDepositorHas ServiceCloning Grade DNAInserthSpCas9n
UseCRISPRTags3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP
Plasmid#48885PurposePositive feedback plasmid from oscillatorDepositorInsertLuxI, sfGFP, and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailabilityAcademic Institutions and Nonprofits only
Showing: 161 - 180 of 8203 results