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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepcDNA3.3
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5'UTR-d2eGFP-3'UTR
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Speciesjellyfish
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Insert Size (bp)1504
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Mutationdestabilized (PEST sequence) GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer TK polyA reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.3_d2eGFP was a gift from Derrick Rossi (Addgene plasmid # 26821 ; http://n2t.net/addgene:26821 ; RRID:Addgene_26821) -
For your References section:
Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ. Cell Stem Cell. 2010 Sep 29. ():. 10.1016/j.stem.2010.08.012 PubMed 20888316