Addgene

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pBKWH-p53

Plasmid

#133900

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (Trp53 Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

T7

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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px330-Axin1

Plasmid

#162543

Purpose

sgRNA targeting Axin1

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Axin1 (Axin1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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MYD88 flag

Plasmid

#13093

Depositor

Ruslan Medzhitov

Insert

MYD88 (Myd88 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-T31N (DN)

Plasmid

#83423

Purpose

Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Residue 31 from T to N (T31N: ACG -> AAC)

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-mWnt1

Plasmid

#176486

Purpose

mouse Wnt1 cDNA in TetO backbone

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

mouse Wnt1 cDNA (Wnt1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TetO

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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Cofilin-pmCherryC1

Plasmid

#27687

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Cofilin (Cfl1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/8R]

Plasmid

#177572

Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/8R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-BDNF-Myc

Plasmid

#124279

Purpose

Express Myc-tagged brain-derived neurotrophic factor (BDNF)

Depositor

Baoji Xu

Article

Xie et al Cell Metab. 2019 Jan 11. pii: S1550-4131

Insert

BDNF (Bdnf Mouse)

Use

AAV

Tags

Myc

Expression

Mutation

Promoter

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

Plasmid

#156432

Purpose

Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

CTCF, rtta3G, TetO3G (Ctcf Mouse)

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-HDR-mEGFP-camk2a

Plasmid

#104589

Purpose

AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2a

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLOVE-miR302/367

Plasmid

#31310

Depositor

Ed Morrisey

Article

Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

Insert

mmu-miR302/367

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-flag-UBR2

Plasmid

#50573

Purpose

Expresses FLAG-tagged mouse UBR2 in mammalian cells.

Depositor

Alexander Varshavsky

Insert

UBR2 (Ubr2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/TagRFP-LL5b

Plasmid

#112828

Purpose

transient/stable overexpression

Depositor

Joshua Sanes, Tomasz Prószyński

Article

Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

Insert

LL5b (Phldb2 Mouse)

Use

Tags

TagRFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAcGP67A - murine TfR

Plasmid

#12392

Depositor

Pamela Bjorkman

Insert

transferrin receptor (Tfrc Mouse)

Use

Tags

6X-His tag, Factor Xa cleavage site, and leader p…

Expression

Insect

Mutation

See comments section

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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eGFP-TRF1 pWzl-Hygro

Plasmid

#19834

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Oct 19. ():.

Insert

Telomere-Repeat binding Factor 1 (Terf1 Mouse)

Use

Retroviral

Tags

enhanced GFP

Expression

Mutation

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pBS mouse c-Myc

Plasmid

#14972

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (Myc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Nucleotides 2578-3294

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-TagRFP658-P2A-GFP

Plasmid

#178971

Purpose

Expresses TagRFP658 and GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

TagRFP658-P2A-GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP beta (LIP)

Plasmid

#12561

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta LIP (Cebpb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
RCAS(A) mdnTcf4Ben (CT#1130)

Plasmid

#14020

Depositor

Cliff Tabin

Article

Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

Insert

Tcf4B (Tcf7l2 Mouse)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Dominant negative Tcf4B, engrailed fusion

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
EF1a_DIO_mMeCP2-HaloTag

Plasmid

#164062

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoter

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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