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Addgene

pAAV-CMV-BDNF-Myc
(Plasmid #124279)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124279 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV-MCS
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 7338
  • Modifications to backbone
    The sequence between two NotI sites is replaced with a sequence containing the CMV promoter, BDNF coding region, Myc tag and BDNF 3' untranslated region.
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BDNF
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3600
  • GenBank ID
    AY057907
  • Entrez Gene
    Bdnf
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-BDNF-Myc was a gift from Baoji Xu (Addgene plasmid # 124279 ; http://n2t.net/addgene:124279 ; RRID:Addgene_124279)
  • For your References section:

    Activation of Anxiogenic Circuits Instigates Resistance to Diet-Induced Obesity via Increased Energy Expenditure. Xie X, Yang H, An JJ, Houtz J, Tan JW, Xu H, Liao GY, Xu ZX, Xu B. Cell Metab. 2019 Jan 11. pii: S1550-4131(18)30755-1. doi: 10.1016/j.cmet.2018.12.018. 10.1016/j.cmet.2018.12.018 PubMed 30661931