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Plasmid#189747PurposeThe construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.DepositorInsertCas9, ALDH1A3 gRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-v2-ALDH1A3gRNA1
Plasmid#189746PurposeThe construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.DepositorInsertCas9, ALDH1A3 gRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-BirA-Linker-Myc-mRap1
Plasmid#184644PurposeRetroviral expression of BirA-mRap1DepositorInsertBirA-rap1
UseRetroviralTagsBirA FLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfCsf1b
Plasmid#168104PurposeExpress soluble His-tagged zebrafish Csf1b in insect cellsDepositorInsertCsf1b (csf1b Zebrafish)
UseTags6x HisExpressionInsectMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-BirA-Linker-Myc-mRap1-I312R
Plasmid#184645PurposeRetroviral expression of BirA Rap1 I312RDepositorInsertBirA-rap1-I312R
UseRetroviralTagsBirA FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DHN_ShDHN (pBS0797)
Plasmid#185260PurposeFor the mammalian expression of the tomato plant protein DHN_ShDHN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertDHN_ShDHN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Grin1 gRNA#3
Plasmid#169791PurposeCas9-mediated knockout of Grin1 in mammalian cellsDepositorInsertGrin1 (Grin1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Grin1 gRNA#2
Plasmid#169790PurposeCas9-mediated knockout of Grin1 in mammalian cellsDepositorInsertGrin1 (Grin1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bmpr2 gRNA#2
Plasmid#163389PurposeCas9-mediated knockout of Bmpr2 in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bmpr2 gRNA#1
Plasmid#163388PurposeCas9-mediated knockout of Bmpr2 in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tbx21 gRNA#2
Plasmid#170838PurposeCas9-mediated knockout of Tbx21 in mammalian cellsDepositorInsertTbx21 (Tbx21 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Limk1 gRNA#3
Plasmid#163393PurposeCas9-mediated knockout of Limk1 in mammalian cellsDepositorInsertLimk1 (Limk1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tbx21 gRNA#3
Plasmid#170839PurposeCas9-mediated knockout of Tbx21 in mammalian cellsDepositorInsertTbx21 (Tbx21 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tbx21 gRNA#1
Plasmid#170837PurposeCas9-mediated knockout of Tbx21 in mammalian cellsDepositorInsertTbx21 (Tbx21 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bmpr2 gRNA#3
Plasmid#163390PurposeCas9-mediated knockout of Bmpr2 in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Limk1 gRNA#2
Plasmid#163392PurposeCas9-mediated knockout of Limk1 in mammalian cellsDepositorInsertLimk1 (Limk1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Grin1 gRNA#1
Plasmid#169789PurposeCas9-mediated knockout of Grin1 in mammalian cellsDepositorInsertGrin1 (Grin1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Venus-Actb
Plasmid#169788PurposeAn actin FRET sensor. Must be expressed with ECFP-actin for FRET.DepositorInsertActb (Actb Mouse)
UseTagsVenusExpressionMammalianMutationPromoterCAGAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAA2
Plasmid#154162PurposeFirefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation16 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA1
Plasmid#154161PurposeFirefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation12 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only