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Plasmid#74614PurposeExpresses a truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-552 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p35S-PBE
Plasmid#119772PurposeConversion of C to T in potatoDepositorInsertNLS-APOBEC1-nCas9-UGI-NLS
UseTagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAGhP14
Plasmid#84362Purposeexpress FLAG-HA tagged human PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseTagsFLAG and HAExpressionMammalianMutationsilent mutations at coding silent 1068CtoT, 1629T…PromoterCAGAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-MAHS
Plasmid#90034PurposeTo express GFP-tagged MAHS in mammalian cells transientlyDepositorInsertMAHS
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV)
Plasmid#140160PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
(+)-Limonene Synthase
Plasmid#112230Purpose(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.DepositorInsert(+)-Limonene Synthase
UseTagsN-terminal His-tagExpressionBacterialMutationdeleted amino acids 1-52PromoterAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHiUGE V5-epitope donor ORF0
Plasmid#200377PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VP64
Plasmid#78901PurposeExpresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertdCas9-VP64
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF2
Plasmid#200379PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
RFP-KIF3A-HL
Plasmid#51397Purposeexpression of RFP-tagged amino acid 369-701 (headless) of KIF3A in mammalian cellsDepositorInsertKIF3A (Kif3a Mouse)
UseTagsRFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC002 - LshC2C2 locus into pACYC184
Plasmid#79151PurposeEndogenous LshC2c2 locus cloned into pACYC184DepositorInsertLshC2c2 locus
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag Jnk3a1
Plasmid#15745DepositorInsertJnk3a1 (MAPK10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
LR (bact: HyPer)
Plasmid#162069PurposeUsed for the diffuse visualization of H2O2 production in cells.DepositorInsertbeta actin 2 (actb2 Zebrafish)
UseGateway donor vectorTagsHyPerExpressionMutationPromoterT3Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-GCN4
Plasmid#78899PurposeExpresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell cultureDepositorInsertdCas9-10XGCN4
UseCRISPRTagsHAExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-OSF-TRIM5-21R
Plasmid#79063PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertOSF-TRIM5-21R
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Mst2
Plasmid#84296PurposeExpresses Myc-tagged Mst2DepositorInsertMst2 (STK3 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-GCaMP6s-P2A-mKate2
Plasmid#112006PurposeFor co-expression of GCaMP6s with mKate2DepositorHas ServiceAAV9InsertGCaMP6s-P2A-mKate2
UseAAVTagsExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only