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Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorInsertFYVE-Hrs x 2 (PIKFYVE Human)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pimEJ5GFP
Plasmid#44026DepositorInsertEJ5GFP egfp-based chromosomal break reporter
UseTagsExpressionMammalianMutationpCAGGS promoter and eGFP separated by pgkPURO cas…PromoterpCAGGSAvailable sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Nst-CAG-DEST
Plasmid#80489PurposeGateway Destination donor vector for AAVS1 targeting (puromycin selection)DepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-F-del Casp9.IRES.GFP
Plasmid#15567DepositorInsertFKBP12(V36)-p30Caspase9 (FKBP1A Human)
UseRetroviralTagsFKBP12 and HA epitopeExpressionMammalianMutationFKBP12 F36 to V Truncated Caspase9 (135-416; lac…PromoterAvailable sinceAug. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
GPR150-Tango
Plasmid#66326PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR150 (GPR150 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-pBAD
Plasmid#54519PurposeLocalization: Protein Expression Vector , Excitation: 485, Emission: 507DepositorTypeEmpty backboneUseTags6xHis and sfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
OTR untagged
Plasmid#208913Purposeencodes the human oxytocin receptor, without any tagsDepositorInsertOXTR (OXTR Human)
UseTagsExpressionMammalianMutationTM5: 5x56, A218, Threonin instead of AlaninPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-YFP
Plasmid#13033PurposeMammalian expression vector for cloning your gene fused to YFPDepositorHas ServiceCloning Grade DNAInsertYellow fluorescent protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x2
Plasmid#58766PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_D210N
Plasmid#111904PurposeExpress D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1DepositorInsertRNASEH1 (RNASEH1 Human)
UseTagsV5ExpressionMammalianMutationChanged D 210 to NPromoterAvailable sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDY0663 ACTB atgRNA with v2 scaffold
Plasmid#179108PurposeACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffoldDepositorInsertACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-His6-2_HBD
Plasmid#171870PurposeExpresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cellsDepositorInsertTandem repeats Hybrid binding domain of Rnase H1
UseTagsGST- and His10-tagExpressionBacterialMutationWTPromoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB
Plasmid#99391PurposeGateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)DepositorTypeEmpty backboneUseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Dead
Plasmid#20859PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).DepositorInsertStreptavidin-Dead
UseTagsExpressionBacterialMutationTriple mutant of wt Streptavidin N23A,S27D,S45APromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur
Plasmid#60507PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-SynTetOff-GFP
Plasmid#190230PurposeGateway entry vector, high-level expression of EGFP in neuronal cellsDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro
Plasmid#89992PurposeDonor template for generation of OCT4-T2A-nEmGFP reporter cell linesDepositorInsertOCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro DEST (w529-2)
Plasmid#19068Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENN226_CRISPRcharm
Plasmid#220834PurposeCRISPRcharm expression in mammalian cellsDepositorInsertCRISPRcharm
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE7.10-NG
Plasmid#181753PurposeMMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE7.10-NG
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCROP-PuroR-P2A-GFP-U6
Plasmid#200029PurposeLentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAsDepositorTypeEmpty backboneUseLentiviralTagsPuroR-P2A-GFPExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-PspCas13b-GEMSgRNA2
Plasmid#204768PurposepU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.DepositorInsertguide RNA targeting GEMS m6A sensor mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM4D(Gi)-mCherry
Plasmid#50475PurposeGi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas ServiceAAV Retrograde, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tol2
Plasmid#31823DepositorInsertTol2 Transposase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-RfxCas13d-His
Plasmid#141322PurposePlasmid for bacterial expression and purification of RfxCas13d proteinDepositorInsertRfx-Cas13d
UseCRISPRTags6xHisExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_8xMmaPylT_EF1_FLAG-MmaPylRS
Plasmid#174890PurposeMma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationU25C PylTPromoterEF1Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorHas ServiceCloning Grade DNAInsertBirA(R118G)-HA
UseTagsHAExpressionMammalianMutationR118G (highly promiscuous form)PromoterAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZR071_SFFV-dCas9-mCherry-KRAB
Plasmid#180264PurposeLentiviral expression vector for dCas9-KRAB in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralTagsKRAB and mCherryExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-BRAF
Plasmid#116719PurposeLentiviral expression of BRAFDepositorInsertBRAF (BRAF Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only