Skip to main content
Addgene

Generating permissive site-specific unnatural aminoacyl-tRNA synthetases.

Miyake-Stoner SJ, Refakis CA, Hammill JT, Lusic H, Hazen JL, Deiters A, Mehl RA
Biochemistry. 2010 Mar 2;49(8):1667-77. doi: 10.1021/bi901947r. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
85482pBad-sfGFPArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag
85483pBad-sfGFP 150TAGArabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150
85484pDule-tfmF A65V S158AMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

Antibodies from Article