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Plasmid#31474DepositorInsertCox8-tagged pHRed
UseTags4xCox8ExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-Neq2X7
Plasmid#182366PurposeExpresses Neq2X7 DNA polymeraseDepositorInsertNeq2X7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LaminB1-10
Plasmid#55069PurposeLocalization: Nuclear Envelope, Excitation: 587, Emission: 610DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB
Plasmid#154473PurposeExpresses dCas9 fused to mCherry and the KRAB domain of ZIM3DepositorInsertZIM3 (ZIM3 Human)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterSFFVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-EF1a-DIO-hM4D(Gi)-mCherry
Plasmid#50461PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsHA and mCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
SepRS(2)/pSertRNA(B4)/EF-Sep
Plasmid#173897PurposeThe plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.DepositorInsertsSepRS(2)
pSertRNA
EF-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_miRFP670
Plasmid#113726PurposeExpresses the fluorescent protein miRFP670 in a lentiviral backboneDepositorInsertmiRFP670
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGD-p19
Plasmid#196326Purpose35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressorDepositorInserttomato bushy stunt virus p19
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B WT
Plasmid#123230PurposeExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Hygro (656-4)
Plasmid#17446Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, HygroDepositorHas ServiceLentiviral PrepInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-Tmem192-2xFlag
Plasmid#102929PurposeExpress a lysosomal TagDepositorInsertTmem192 (TMEM192 Human)
UseLentiviralTagsFlagExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR7-YFP
Plasmid#13022DepositorInsertTLR7 (TLR7 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-UBnc-PURO
Plasmid#208044PurposeEnables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycinDepositorInsertUBnc (UBC Human)
UseLentiviralTagsLow-affinity AvitagExpressionMutationL73P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV mGAD65(delE1) GFP WPRE SV40pA
Plasmid#177316PurposeTo produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.DepositorInsertmGAD65(delE1) promoter, GABAergic interneurons specific promoter
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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BPK1520
Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD63-pHluorin
Plasmid#130901PurposeExpression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD63-pHluorin (CD63 Human, Aequorea victoria)
UseTagspHluorinExpressionMammalianMutationpHluorin inserted between Gln-36 and Leu-37 in ex…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX_DSB_Spectrum-V1
Plasmid#192474PurposeLentiviral DSB Spectrum V1 Reporter SystemDepositorInsertBFP1.2, incompleteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-GCaMP6s
Plasmid#135418PurposeCan be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.DepositorInsertGCaMP6s
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only