Skip to main content
Addgene
Showing: 3441 - 3480 of 149050 results

  1. CMV-SEAP

    Plasmid
    #24595
    Depositor
    Alan Cochrane
    Insert
    SEAP (ALPP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 23, 2010
    Availability
    Academic Institutions and Nonprofits only
    24595-plasmid-map-sequence-id-260470

    Enlarge

  3. pFGL821-8

    Plasmid
    #58224
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    hygromycin B phosphotransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    May 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    58224-plasmid-map-sequence-id-89706

    Enlarge

  7. pComb3XSS

    Plasmid
    #63890
    Purpose
    3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Inserts
    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    Use
    Phage display
    Tags
    6x His, HA tag, and geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63890-plasmid-map-sequence-id-372937

    Enlarge

  10. mEmerald-HP1-10

    Plasmid
    #54124
    Purpose
    Localization: Heterochromatin, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    HP1 (CBX5 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. CSNK1G1

    Plasmid
    #38934
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CSNK1G1 (CSNK1G1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid
    #111573
    Purpose
    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells
    Depositor
    Thomas Muir
    Article
    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038
    Insert
    PARP1 (PARP1 Human)
    Use
    Tags
    C-terminal 6xHis and C-terminal Flag
    Expression
    Insect
    Mutation
    lysine to glutamine mutation at the following ami…
    Promoter
    polyhedrin promoter
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111573-plasmid-map-sequence-id-215588

    Enlarge

  19. mTagBFP-Cx43-7

    Plasmid
    #55256
    Purpose
    Localization: Gap Junctions, Excitation: 399, Emission: 456
    Depositor
    Michael Davidson
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    mTagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55256-plasmid-map-sequence-id-209197

    Enlarge

  20. mEGFP-NbALFA

    Plasmid
    #159986
    Purpose
    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)
    Depositor
    Motoyuki Hattori
    Article
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    mEGFP-NbALFA
    Use
    Tags
    8histidine-HRV3C protease cleavage site
    Expression
    Bacterial
    Mutation
    3GS linker between mEGFP and NbALFA
    Promoter
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159986-plasmid-map-sequence-id-314744

    Enlarge

  21. pCMV-SUN2 mScarlet

    Plasmid
    #187685
    Purpose
    In vivo visualization of SUN2
    Depositor
    Mary Beckerle
    Insert
    SUN2 (Sun2 Mouse)
    Use
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    187685-plasmid-map-sequence-id-371432

    Enlarge

  23. mTagRFP-T-Zyxin-6

    Plasmid
    #58031
    Purpose
    Localization: Focal Adhesions, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    Zyxin (ZYX Human)
    Use
    Tags
    mTagRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    58031-plasmid-map-sequence-id-198672

    Enlarge

  24. pGBW-m4134452

    Plasmid
    #152327
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152327-plasmid-map-sequence-id-317257

    Enlarge

  25. pCW57.1 N-term GFP tTA

    Plasmid
    #107551
    Purpose
    All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.
    Depositor
    David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP (optional)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107551-plasmid-map-sequence-id-208339

    Enlarge

  29. Lifeact-Electra2

    Plasmid
    #218975
    Purpose
    In vivo visualization of actin cytoskeleton
    Depositor
    Amro Hamdoun
    Insert
    Lifeact-Electra2
    Use
    Tags
    Electra2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218975-plasmid-map-sequence-id-433880

    Enlarge

  35. qTAG-N-Blast-V5

    Plasmid
    #207686
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207686-plasmid-map-sequence-id-413750

    Enlarge

  37. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201152
    Purpose
    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    dCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mutation
    gRNA sequence: TACGTTCTCTATCACTGAT
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201152-plasmid-map-sequence-id-401799

    Enlarge

  38. TFORF1637

    Plasmid
    #143830
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HOXC12 (HOXC12 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143830-plasmid-map-sequence-id-396271

    Enlarge

Showing: 3441 - 3480 of 149050 results