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Plasmid#227903PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227904PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227905PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCOPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227890PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertTOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-CASK
Plasmid#221427PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertCASK (CASK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-ΔPR1
Plasmid#214902Purposeexpression of the truncated variants of the SHIP2 without first proline-rich domainDepositorInserthuman SHIP2 delta aa 123-410 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 123-410aaPromoterCMVAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-C2
Plasmid#214906Purposeexpression of the truncated variants of the SHIP2 that retains second proline-rich domainDepositorInserthuman SHIP2 aa 740-1189 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 1-739, 1190-1258aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-C1
Plasmid#214905Purposeexpression of the truncated variants of the SHIP2 that retains second proline-rich domain and C-terminal sterile alpha motifDepositorInserthuman SHIP2 aa 740-1258 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 1-739aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-N2
Plasmid#214904Purposeexpression of the truncated variants of the SHIP2 that retains first proline-rich domainDepositorInserthuman SHIP2 aa 123-418 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 1-122, 419-1258aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-N1
Plasmid#214903Purposeexpression of the truncated variants of the SHIP2 that retains only SH2 domain and first proline-rich domainDepositorInserthuman SHIP2 aa 1-418 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 419-1258aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only