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pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

Plasmid

#171428

Purpose

Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

FKBP-(GGS)X6-HaloTag C58

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(S317A)-EGFP

Plasmid

#89636

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Mutation

S317A, AGC-->GCC

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

Plasmid

#86885

Purpose

C-terminal part of the split-BioID

Depositor

Mathieu Bollen

Article

Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

Insert

BirA* (AA141-321)- HA- NIPP1]

Use

Tags

HA tag

Expression

Mammalian

Mutation

BirA Amino Acids 141-321

Promoter

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

Plasmid

#201217

Purpose

pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Use

Tags

Flag and SNAP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

Plasmid

#171427

Purpose

Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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(133) pcDNA3.1-Nup62-Flag-EPEA

Plasmid

#160744

Purpose

Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

Nup62-Flag-EPEA

Use

Tags

EPEA and Flag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Linear Tau 0N4R 3X Flag tag

Plasmid

#194171

Purpose

Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-Associated Protein Tau 0N4R

Use

Tags

3X Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

Plasmid

#158953

Purpose

Express human RAD51(T309A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Use

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed threonine 309 to alanine

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-FKBP-Myc

Plasmid

#177908

Purpose

human CD81-TEV cleavage site-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81 (CD81 Synthetic, Human)

Use

Tags

FKBP, Myc tag, and TEV protease cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) L533E V5 His

Plasmid

#119288

Purpose

CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Use

Tags

V5 His

Expression

Mammalian

Mutation

L533E

Promoter

T7

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-SIV-RCM-vpx

Plasmid

#115844

Purpose

Encodes codon optimized FLAG tagged SIVRCM-Vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM-Vpx

Use

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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XE248 pCDNA3.1+zeo-VSV-KLHL12

Plasmid

#16761

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

pCDNA3.1+zeo-VSV-KLHL12 (KLHL12 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A HIS-TEV-DPP9S

Plasmid

#166791

Purpose

Mammalian cell expression vector for His-tagged DPP9S

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

HIS-TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG S297A

Plasmid

#169949

Purpose

Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p190-FLAG

Plasmid

#205620

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p190 isoform b3a2

Use

Tags

FLAG

Expression

Mammalian

Mutation

T117M- Please see depositor comments

Promoter

CMV

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) Full length Her2(ERBB2)-Fc

Plasmid

#194330

Purpose

Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein

Depositor

Alexander McLellan

Insert

Her2-Fc (ERBB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/nV5-DEST-beta catenin

Plasmid

#20140

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

T3I

Promoter

CMV

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

Plasmid

#172647

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

Plasmid

#13005

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 delta CC (SULF1 Human)

Use

Tags

Expression

Mammalian

Mutation

changed Cysteine 87 to Alanine

Promoter

Available since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
NC3 pCDNA3.1 FLAG WTX (1-804)

Plasmid

#36958

Depositor

Randall Moon

Article

Major et al Science. 2007 May 18;316(5827):1043-6.

Insert

WTX (AMER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only