(133) pcDNA3.1-Nup62-Flag-EPEA
(Plasmid
#160744)
-
PurposeExpresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 160744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1(+)
-
Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 5371
- Total vector size (bp) 6988
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNup62-Flag-EPEA
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1617
- Promoter T7/CMV
-
Tags
/ Fusion Proteins
- Flag (C terminal on insert)
- EPEA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sgfl (unknown if destroyed)
- 3′ cloning site Sgsl (unknown if destroyed)
- 5′ sequencing primer T7 fwd
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
(133) pcDNA3.1-Nup62-Flag-EPEA was a gift from Christina Woo (Addgene plasmid # 160744 ; http://n2t.net/addgene:160744 ; RRID:Addgene_160744) -
For your References section:
Engineering a Proximity-Directed O-GlcNAc Transferase for Selective Protein O-GlcNAcylation in Cells. Ramirez DH, Aonbangkhen C, Wu HY, Naftaly JA, Tang S, O'Meara TR, Woo CM. ACS Chem Biol. 2020 Apr 17;15(4):1059-1066. doi: 10.1021/acschembio.0c00074. Epub 2020 Mar 2. 10.1021/acschembio.0c00074 PubMed 32119511