Showing: 1681 - 1700 of 13889 results
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Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
UseTagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialMutationPromoterT5AvailabilityAcademic Institutions and Nonprofits only -
KBB394
Plasmid#185086PurposeNMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNma111 KKR 9-11 AAA; KRK 28-30 AAAPromoterAvailabilityAcademic Institutions and Nonprofits only -
KBB441
Plasmid#185131PurposeSwi6 full length with L->A mutated in nuclear localization signalDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSWI6 deletion of amino acids 450-458PromoterAvailabilityAcademic Institutions and Nonprofits only -
AAV-H2GH
Plasmid#182202PurposeAdeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion constructDepositorInserthistone2B-HaloTag-GFP
UseAAVTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-ZmAFB2/3 a
Plasmid#188423PurposeZea Mays Nuclear Auxin Signaling Receptor AFB2/3 ADepositorInsertAuxin Signaling Receptor AFB2/3 a
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-ZmAFB2/3 b2
Plasmid#188425PurposeZea Mays Nuclear Auxin Signaling Receptor AFB2/3 b2DepositorInsertAuxin Signaling Receptor AFB2/3 b2
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pENTR-ZmIAA8ΔPB1
Plasmid#188432PurposeZea Mays Nuclear Auxin Signaling Repressor IAA8DepositorInsertAuxin Signaling Repressor IAA8deltaPB1
UseCloningTagsExpressionMutationdeleted PB1 domainPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGP4G-REL2N91-ccdB
Plasmid#188444PurposeZea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDESTDepositorInsertREL2N91/ccdB
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Cyto-mTurq2-Apollo-NADP+
Plasmid#193301PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasmDepositorInsertG6PD
UseTagsmTurqoise2ExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Tol2_B-act_dCas9-Vp64-T2A-Citrine
Plasmid#200248PurposeZebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesisDepositorInsertdCas9-Vp64-T2A-Citrine
UseZebrafish expressionTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
M-NylC_pET28a
Plasmid#215409PurposeExpression construct for M-NylC gene, codon optimized for expression in E. coliDepositorInsertM-NylC
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCL1032
Plasmid#217962PurposeMammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.DepositorInsertNASP (NASP Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
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pK7WGF2::hCas9
Plasmid#46965PurposeExpesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissueDepositorInserthCas9
UseCRISPR; Plant expressionTagseGFPExpressionMutationPromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
Plasmid#45579PurposeExpresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani K227M
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
p1553 CMV-EBNA1 (delta GGA)
Plasmid#26648DepositorInsertEpstein-Barr Nuclear Antigen 1 (EBNA-1 Epstein-Barr Virus)
UseTagsExpressionMammalianMutationdeletion of 224 amino acids of EBNA-1 (aa101-324 …PromoterAvailabilityAcademic Institutions and Nonprofits only -
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pMT1100
Plasmid#8618DepositorInsertribonuclease Sa3, barstar
UseTagsphoA signal sequence to RNase Sa3ExpressionBacterialMutationIn addition to the substitution of the barnase ge…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry::LANS1
Plasmid#60784PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear TranslocationDepositorInsertLANS1
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1681 - 1700 of 13889 results