Addgene

We narrowed to 3,755 results for: pCMV

Showing: 1661 - 1680 of 3755 results

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pCMV-Tag4A-MMP8 (P78S)

Plasmid

#29546

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

P78S

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCMV6-XL4 FLAG-TM9SF4-Fc

Plasmid

#115770

Purpose

Expression of the extracellular domain of TM9SF4 with N-terminal FLAG tag and C-terminal hIgG tag.

Depositor

Davide Comoletti

Insert

TM9SF4 (TM9SF4 Human)

Use

Tags

FLAG and hIgG/Fc

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-S384A

Plasmid

#33070

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S384A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Tag 3A/myc-M1L195V

Plasmid

#87716

Purpose

Expresses myc-M1L195V spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; L195V

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1-IRES2-EGFP

Plasmid

#170820

Purpose

human GABAA receptors alpha1 subunit and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit (GABRA1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3-Y20A

Plasmid

#58460

Purpose

Expresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 to alanine

Promoter

CMV IE

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
pCMV6-XL4 FLAG-TMEFF2-Fc

Plasmid

#115774

Purpose

Expression of the extracellular domain of TMEFF2 with N-terminal FLAG tag and C-terminal hIgG tag.

Depositor

Davide Comoletti

Insert

TMEFF2 (TMEFF2 Human)

Use

Tags

FLAG and hIgG/Fc

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_RBMX-eA3A-UdgX-HF-nCas9

Plasmid

#163548

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-HF-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-rat ERK2-S151D

Plasmid

#40818

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

S151D

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock L57E

Plasmid

#47337

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

L57E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock L74E

Plasmid

#47338

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

L74E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCMV5-FLAG-MKK6-K82M

Plasmid

#21583

Depositor

John Kyriakis

Article

Yuasa et al J Biol Chem. 1998 Aug 28. 273(35):22681-

Insert

MKK6 (MAP2K6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

lysine 82 to methionine

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN-W440L

Plasmid

#73377

Purpose

Myc tagged human XPLN expression with a point mutation W440L

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Use

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

W440L

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN-L269E

Plasmid

#73376

Purpose

Mammalian expression of mutant XPLN (Arhgef3) gene Leu269 --> Glu

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Use

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

L269E

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 S129N

Plasmid

#51587

Purpose

expresses RNAiR myc-tagged Rem2 non-GTP binding

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

S129N; silent mutations at shRNA 1 and 2 recognit…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 L317G

Plasmid

#51586

Purpose

expresses RNAiR myc-tagged Rem2 non-CaM interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

L317G; silent mutations at shRNA 1 and 2 recognit…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-rat ERK2-L73P

Plasmid

#40817

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

L73P

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
p224 pCMV-RFP/CREM

Plasmid

#8401

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

RFP/CREM

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

modified Cre, LOX 511 sites surrounding RFP withi…

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S381A

Plasmid

#33090

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Tags

Flag

Expression

Mammalian

Mutation

S381A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only