pCMV5-rat ERK2-L73P
(Plasmid
#40817)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 40817 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
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Backbone manufacturerDavid Russell, UTSW
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 6200
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMAPK1
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Alt nameERK2
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Alt namep42
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1500
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MutationL73P
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GenBank IDNM_053842
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Entrez GeneMapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
- Promoter CMV
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMelanie Cobb, UTSW
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV5-rat ERK2-L73P was a gift from Natalie Ahn (Addgene plasmid # 40817 ; http://n2t.net/addgene:40817 ; RRID:Addgene_40817) -
For your References section:
Constitutive activation of extracellular signal-regulated kinase 2 by synergistic point mutations. Emrick MA, Hoofnagle AN, Miller AS, Ten Eyck LF, Ahn NG. J Biol Chem. 2001 Dec 7;276(49):46469-79. Epub 2001 Oct 8. 10.1074/jbc.M107708200 PubMed 11591711