Showing: 1661 - 1680 of 12934 results
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Plasmid#81184PurposeExpresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA
Plasmid#81185PurposeExpresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5cDepositorInsertsCas5c
Cas8c
Cas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris sp2 CRISPR/pACYCDuet-1
Plasmid#81186PurposeExpresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coliDepositorInsertSynthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
UseCRISPRTagsNoneExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-K
Plasmid#81193PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below., Please view…PromoterROX3 and SNP52pAvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailabilityAcademic Institutions and Nonprofits only -
All_in_one_CRISPR/Cas9_LacZ
Plasmid#74293Purpose"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screeningDepositorInsertsLacZ-alpha
Cas9
mCherry
UseCRISPRTagsHAExpressionBacterial and MammalianMutationPromoterLac promoter, SV40, and T7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pMOD6-pGRR-r11-r10-yeGFP
Plasmid#74368PurposepGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.DepositorArticleInsertyeGFP
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterpGRR-r11-r10AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-iRGR-r11
Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r11
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAHD1AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r10
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterADH1AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA1-gRNA_A
Plasmid#74374PurposegRNA_A to knockout human AMPK alpha 1 using Cas9nDepositorInserthuman AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA1-gRNA_B
Plasmid#74375PurposegRNA_B to knockout human AMPK alpha 1 using Cas9nDepositorInserthuman AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_A
Plasmid#74376PurposegRNA_A to knockout human AMPK alpha 2 using Cas9n.DepositorInserthuman AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_B
Plasmid#74377PurposegRNA_B to knockout human AMPK alpha 2 using Cas9nDepositorInserthuman AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR_v2
Plasmid#74407PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR (ANV)_v2
Plasmid#74408PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pUC57-sgDedd-1
Plasmid#74435PurposesgRNA expression vector for mouse Dedd gene targeting intron 5DepositorInsertDedd
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUC57-sgDedd-2
Plasmid#74436PurposesgRNA expression vector for mouse Dedd gene targeting intron 4DepositorInsertDedd
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSG4K5
Plasmid#74492PurposeFor cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119AvailabilityAcademic Institutions and Nonprofits only
Showing: 1661 - 1680 of 12934 results