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Plasmid#204398PurposeA pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRpa0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only
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pMZ3012
Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRhl0L0L12Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL70008
Plasmid#50336DepositorInsertpFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET:GACT:GFP
Plasmid#60734PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-T43NTR-TrxA-H10
Plasmid#121677PurposeBacterial expression of neurotensin receptor fusion proteinDepositorInsertneurotensin receptor (Ntsr1 Rat)
UseTagsTrxA-H10ExpressionBacterialMutationdeleted amino acids 1-42PromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-Smad1 (AAVA)
Plasmid#14957DepositorInsertSmad1 (AAVA) (SMAD1 Human)
UseTagsFlagExpressionMammalianMutationThree Serine to Alanine mutations in the C-termin…PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL3-LRR
Plasmid#66937PurposeDestination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Reverse orientation (attR2, attR1).DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterSV-40Available sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHygroR-GMRWhite
Plasmid#165886PurposeHygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICH87633
Plasmid#50271DepositorInsertpromoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONRPEX18Gm-SceI-pheS
Plasmid#111609PurposeGateway compatible allelic exchange vector for Burkholderia sp.DepositorTypeEmpty backboneUseAllelic exchange in burkholderia sp.TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH-HHT2
Plasmid#86482Purposeyeast expression of HHT2 with His10-TEV-HA tagDepositorInsertHHT2 (HHT2 Budding Yeast)
UseTagsHIS10-TEV-HA (HTH)ExpressionYeastMutationPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCI-HA-mAVO3 (pRL62-3)
Plasmid#39210DepositorInsertmAVO3 (Rictor Mouse)
UseTagsHAExpressionMammalianMutationG5S and I15VPromoterCMVAvailable sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A human LRP6 E1E2 (20–629)
Plasmid#132627Purposeexpresses human LRP6 E1E2 (20–629) in Hi5 insect cellDepositorInsertLRP6 (20–629)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBR322 14-3-3 Promoter
Plasmid#16517DepositorInsert14-3-3 promoter (SFN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pICSL50015
Plasmid#50318PurposePlease note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)DepositorInsertCDS, mNEON (Branchistoma lanceolatum)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD)
Plasmid#176191PurposegfcD protein expressionDepositorInsertgfcD
UseTags6xHisExpressionBacterialMutationPromoterPtacAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD-10xHis)
Plasmid#176192PurposegfcD protein expressionDepositorInsertgfcD
UseTags10xHisExpressionBacterialMutationPromoterPtacAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG26(gfcD)
Plasmid#176198PurposegfcD protein expressionDepositorInsertgfcD
UseTags10xHisExpressionBacterialMutationPromoterT7Available sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcBCD)
Plasmid#176193Purposeexpresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operonDepositorInsertgfcBCD
UseTagsExpressionBacterialMutationPromoterPtacAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA
Plasmid#188704PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2DepositorInsertNFATC2 sgRNAs
UseLentiviralTagsExpressionMammalianMutationPromoterhUbCAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only