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pMuLE Lenti Dest Luc2

Plasmid

#62179

Purpose

MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses firefly luciferase under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Luciferase; Mule gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Del-7

Plasmid

#14968

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-26/+334) (MYC Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-26/+334 (relative to the P2 transcription initia…

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
LB360 (Bcl-2 from -1287 to -1337)

Plasmid

#15242

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -1337 with P1

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pGL3-CMV-Cdc42 3'UTR

Plasmid

#51389

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

Cdc42 3'UTR (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pIS1 DICER1 long-mut-let-7

Plasmid

#21651

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant let-7 microRNA binding sites

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-GNB2 5'-UTR

Plasmid

#115355

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of GNB2

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

GNB2 5'-UTR (GNB2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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psiCheck-DNAJA4CDS

Plasmid

#41849

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

DNAJA4 CDS (DNAJA4 Human)

Use

Luciferase

Tags

renilla luciferase

Expression

Mutation

Start codon of DNAJA4 removed

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pSB165 - pL1R3_pMAS::rLUC-I::tMAS

Plasmid

#123198

Purpose

binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

pMAS::rLUC-I::tMAS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-all

Plasmid

#21653

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated let-7 and miR103/107 microRNA binding sit…

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-miR103/107

Plasmid

#21652

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant miR103/107 microRNA binding sites

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Frag-F

Plasmid

#14053

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/-3) (MYC Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-109/-3 (relative to the P2 transcription initiat…

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
LB1361 (Bcl-2 promoter with MHS12)

Plasmid

#15391

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Bcl-2-Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with MHS12

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

Plasmid

#127308

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRP_8xgRNA-FLuc

Plasmid

#135937

Purpose

Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

minCMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127316

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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Psicheck2 MERTK full length 3'UTR

Plasmid

#26992

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

MERTK Full length 3' UTR (MERTK Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127318

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCheck-ApoECDS

Plasmid

#41848

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

ApoE CDS (APOE Human)

Use

Luciferase

Tags

renilla luciferase

Expression

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
PCD FL1P

Plasmid

#12567

Depositor

Carl Novina

Article

Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Insert

One perfect CXCR4 binding site (CXCR4 Human)

Use

Luciferase; Reporter for translation repression

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pIS1 IMP-1 long-mut-let-7

Plasmid

#21642

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant let-7 microRNA binding sites

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge