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Plasmid#22595DepositorInsertUSP16 (USP16 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSR657
Plasmid#65214PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-2 cap
UseTagsExpressionInsectMutationPromoterp10 and polyhedrinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP
Plasmid#10676DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadDE
Plasmid#193837PurposeExpress TadDE (with SpCas9) in mammalian cellsDepositorInsertTadA-DualEditor-Cas9D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0033 Ef1a-Pa01-2a-EGFP
Plasmid#193460PurposeExpression of RecombinaseDepositorInsertPa01
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_WKKD
Plasmid#111905PurposeExpress WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybridsDepositorInsertRNASEH1 (RNASEH1 Human)
UseTagsV5ExpressionMammalianMutationChanged D 210 to N, W 43 to A, K 59 to A, K 60 to…PromoterAvailable sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGM1190
Plasmid#69994PurposeOriT containing mobilizable vector for thiostrepton induced gene expression in StreptomycesDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_AAVS1_T2
Plasmid#79888PurposeExpresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.DepositorTypeEmpty backboneUseCRISPRTagsUntagged eSpCas9(1.1)ExpressionMammalianMutationPromoterCBhAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDest-527
Plasmid#11518PurposeGateway destination vector for bacterial expression; His tagDepositorTypeEmpty backboneUseGateway destination vectorTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-ApoE2
Plasmid#87085PurposeExpresses ApoE2 in Mammalian cellsDepositorInsertApolipoprotein E2 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.ffLuciferase.SV40
Plasmid#105532PurposeAAV expression of firefly luciferase from CMV promoterDepositorHas ServiceAAV8InsertLuciferase
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-NanoLuc
Plasmid#188116PurposeTemplate plasmid to transcribe circular RNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast-SCRAMBLE
Plasmid#26701Purpose3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selectionDepositorInsertscramble
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Raichu-RhoA-CR
Plasmid#40258DepositorInsertRaichu-RhoA-CR (RHOA Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
LSL-Cas9-Rosa26TV
Plasmid#61408PurposeCre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.DepositorInsertsCas9
EGFP
UseCRISPR, Cre/Lox, and Mouse TargetingTags3xFLAG and P2AExpressionMammalianMutationhuman codon optimizedPromoterCAGAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-FLAG-MLL-AF9
Plasmid#71444PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2DepositorInsertMLL-AF9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-beta-3
Plasmid#27289DepositorInsertintegrin beta 3 (ITGB3 Human)
UseTagsHis and MycExpressionMammalianMutationn/aPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP33
Plasmid#22601DepositorInsertUSP33 (USP33 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo-hTERT
Plasmid#1774PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBI-MCS-EGFP
Plasmid#16542DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only