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Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-V5-hphMX4
Plasmid#20779DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pZR060_CROPseq-sgCtrl-CRISPRi
Plasmid#180269PurposeLentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRiDepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-N108Q
Plasmid#194157PurposeMammalian expression of ABE8e-N108QDepositorInsertTadA-8e (N108Q)-nSpCas9
UseCRISPRTags6*HisExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGreenFire1_E2F1RE
Plasmid#112248Purposeexpression of GFP and Luciferase under the control of E2F1 responsive elementDepositorInsertE2F1RE (responsive element)
UseLentiviralTagscopGFP and luciferaseExpressionMutationPromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDACR3882 [rab-3p::HYlight::let-858utr]
Plasmid#207666PurposeExpresses worm-optimized HYlight in C. elegans neuronsDepositorInsertHYlight Biosensor
UseTagsExpressionWormMutationPromoterrab-3Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDACR3883 [rab-3p::HYlight-RA::let-858utr]
Plasmid#207667PurposeExpresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neuronsDepositorInsertHYlight-RA [T152E]
UseTagsExpressionWormMutationPromoterrab-3Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-NCAM-1
Plasmid#205190PurposeThis vector encodes of a synCAM (NCAM1) and GFPDepositorInsertsynCAM NCAM1 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP‐RNASEH1
Plasmid#108699PurposeFor expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cellsDepositorInsertRNASEH1 (nuclear isoform) (RNASEH1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -