pLexM-SLC3A2
(Plasmid #197994)

• Purpose: Expresses SLC3A2 as an N-terminal His tagged construct in mammalian cells
• Depositing Lab: Simon Newstead
• Publication: Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi: 10.1038/s41467-021-27414-1.

Backbone

• Vector backbone: pLexM
• Backbone manufacturer: Plasmid 99844
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLC3A2
• Alt name: 4f2hc
• Species: H. sapiens (human)
• Insert Size (bp): 1731
• Mutation: Please see depositor comments below
• GenBank ID: J02939.1 J02939.1
• Entrez Gene: SLC3A2 (a.k.a. 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE)
• Promoter: CMV
• Tag / Fusion Protein:
  • His tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV_F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a P539S mutation in SLC3A2. This mutation is not known to affect plasmid function.