Addgene

Showing: 1581 - 1600 of 2985 results

Results per page:

Sort results by:

pCAG-EGFP/RFP-miR-Scrint

Plasmid

#19821

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

Scrambled miRNA

Use

Cre/Lox and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-miRFP720-P2A-GFP

Plasmid

#197165

Purpose

Expresses the protein of miRFP720-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmGFP-P2A-K(AAA)12-P2A-RFP

Plasmid

#105687

Purpose

Double fluorescent stalling reporter K(AAA)12

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)12
P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mIRFP670nuc-TEV-Xph20-ETWV

Plasmid

#133044

Purpose

Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Use

Tags

NLS, TEV protease + TEV cleavage site, and miRFP6…

Expression

Mammalian

Mutation

S63K

Promoter

CAG

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190077

Purpose

express human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2, TMPRSS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-miRFP670-3XNLS-WPRE

Plasmid

#159952

Purpose

nucleus marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

miRFP670-3XNLS

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMVtight-UPRT-T2A-RFP-IRES-CD

Plasmid

#126677

Purpose

Doxycycline inducible expression of UPRT and CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)

Use

Tags

Expression

Mutation

Promoter

tight TRE

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-T2A-Gal80-3xP3-RFP

Plasmid

#127562

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal80

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS6-ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB

Plasmid

#111504

Purpose

Expresses a zinc finger specific to the human VEGFA locus, which is linked to a KRAB transcriptional repression domain via a StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

Depositor

Michael Lin

Article

Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

Insert

ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB

Use

Tags

Expression

Mammalian

Mutation

The HCV NS3 protease carries F43L, Q80K, S122R, a…

Promoter

CMV

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS2CP(V29I)-myc-T2A-tagRFP

Plasmid

#140260

Purpose

Expresses MS2CP(V29I) in mammalian cells

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

Coat protein of bacteriphage MS2

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG

Plasmid

#168998

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Unspecified

Tags

EGFP and mRFP1

Expression

Mutation

Promoter

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV[Exp]-Puro-EF-1α>{H2B-RFP}

Plasmid

#201121

Purpose

RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

No

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-T2A-Gal4DBD-3xP3-RFP

Plasmid

#127564

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal4DBD

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-T2A-LexGAD-3xP3-RFP

Plasmid

#127558

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

LexGAD

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-T2A-p65-3xP3-RFP

Plasmid

#127565

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

p65

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-T2A-QF2-3xP3-RFP

Plasmid

#127559

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

QF2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMT-PT2A-GFP-Tdtomato-iRFP670

Plasmid

#111812

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

GFP
Tdtomato
iRFP670

Use

Cloning intermediate

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-T2A-sfGFP-3xP3-RFP

Plasmid

#127557

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sfGFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR pUAS-dGFP pPGK-iRFP

Plasmid

#176530

Purpose

Lentiviral vector expressing a UAS-driven destabilized GFP, as well as a PGK promoter-driven iRFP marker.

Depositor

Jared Toettcher

Article

Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

Inserts

dGFP
iRFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGKp and UASp

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC588 - pAAV c-fos Nuc-iRFP

Plasmid

#59132

Purpose

An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Infrared Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

mFos

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge