Showing: 141 - 160 of 3061 results
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Plasmid#46759PurposegRNA cloning vector for in vitro transcription of target gRNADepositorInsertgRNA core
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCS2(+)-zGrad
Plasmid#119716PurposePlasmid to generate zGrad mRNA to inactivate proteins in zebrafishDepositorInsertzGrad
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:mCD8mCherry
Plasmid#170207PurposeDrives mCD8mCherry expression with the slc1a3b promoter in astrocytesDepositorInsertmCD8
UseZebrafishTagsmCherryExpressionMutationPromoterslc1a3bAvailabilityAcademic Institutions and Nonprofits only -
pDONR221-psmg1
Plasmid#192723PurposeGateway entry vector encoding zebrafish psmg1DepositorInsertpsmg1 (psmg1 Zebrafish)
UseGateway entry vectorTagsExpressionMutationR96Q (rs514814252); T152M (rs501951686); T189A (r…PromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pDONR221-usp25
Plasmid#192733PurposeGateway entry vector encoding zebrafish usp25DepositorInsertusp25 (usp25 Zebrafish)
UseGateway entry vectorTagsExpressionMutationS403R; W622R; 725A_726AinsAA (rs508161559); A730T…PromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pDONR221-zgc:152904
Plasmid#192740PurposeGateway entry vector encoding zebrafish zgc:152904DepositorInsertzgc:152904 (zgc:152904 Zebrafish)
UseGateway entry vectorTagsExpressionMutationN56D; 663T_664DinsA; 733V_772Edel (exon 24)PromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9-T2A-GFP
Plasmid#63155PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.DepositorInsertCas9-T2A-GFP
UseCRISPR; Tol2 middle entry vector for gatewayTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:ChR2(H134R)-tdTomato
Plasmid#124237PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomatoDepositorInsertChR2(H134R)-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:SPRED1
Plasmid#118851PurposeExpresses human SPRED1 and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertSPRED1 (SPRED1 Human, Zebrafish)
UseZebrafish expression, tol7TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
th-P2A-EGFP donor
Plasmid#65562PurposeIntron targeting-mediated EGFP knockin at the zebrafish th locus.DepositorInsertth-P2A-EGFP (th Zebrafish)
UseZebrafish injectionTagsExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
th-P2A-Gal4 donor
Plasmid#65563PurposeIntron targeting-mediated Gal4 knockin at the zebrafish th locus.DepositorInsertth-P2A-Gal4 (th Zebrafish)
UseZebrafish injectionTagsExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-4kb wnt3 promoter-Fzd1mApple
Plasmid#203795PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesisDepositorInsertFzd1 (fzd1 Zebrafish)
UseZebrafish expressionTagsmAppleExpressionMutationPromoter4kb Wnt3 promoterAvailabilityAcademic Institutions and Nonprofits only -
acta1-Cavin1a-Clover
Plasmid#163852PurposeZebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPRDepositorInsertCavin1a (cavin1a Zebrafish)
UseZebrafish expressionTagsCloverExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDONR221-nrip1b
Plasmid#192732PurposeGateway entry vector encoding zebrafish nrip1bDepositorInsertnrip1b (nrip1b Zebrafish)
UseGateway entry vectorTagsExpressionMutationL661P (rs511527097); S720P; A801T; K862E; A863P; …PromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pAWO-SUMO-3C_14M_zTDP2
Plasmid#200512PurposeExpression of zebrafish TDP2 recombinant proteinDepositorInsertTDP2 (tdp2b Zebrafish)
UseTagsHIS-SUMOExpressionBacterialMutationL136N, A139S, C239T, K240R, K309Q, K314L, T315G, …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pHD066
Plasmid#84032PurposeVector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafishDepositorInsertsCre
Venus
UseCre/Lox; Zebrafish transgenesisTagsmCherryExpressionMutationPromoterCrystalin and ElastaseAvailabilityAcademic Institutions and Nonprofits only -
pKHR7
Plasmid#74624Purposea cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.DepositorInsertcmlc2::mCherry
UseZebrafish expressionTagsExpressionMutationPromotercmlc2AvailabilityAcademic Institutions and Nonprofits only -
pT2-eab2-[GTR]
Plasmid#46144DepositorInsertEGFP/mCherry
UseCre/Lox; Tol2 zebrafish transgenic expressionTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTol-Promoter:NLS-tdMCP-eGFP
Plasmid#86248PurposeFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterDepositorInsertbeta Actin promoter:NLS-tdMCPeGFP
UseUsed with transposase to make transient or stable…TagsNLS-tdMCP-eGFPExpressionMutationPromoterBeta ActinAvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-Vector-Dendra2
Plasmid#97101Purposeempty vector for overexpression of microRNA and Dendra2 in ZebrafishDepositorTypeEmpty backboneUseZebrafishTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 141 - 160 of 3061 results