Showing: 141 - 160 of 591 results
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Plasmid#149390PurposeP. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation5bp inserted downstream of the IHFprox sitePromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D
Plasmid#149391PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation10bp inserted downstream of the IHFprox sitePromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Opt
Plasmid#149395PurposeP. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHFprox site is optimized at key positions needed…PromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IRdist_scram
Plasmid#162319PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationThe distal Upstream Motif site is scrambledPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pgRNAkan-glpR
Plasmid#169873PurposeSingle guide RNA plasmid for Cas9 targeting glpR in P. putidaDepositorInsertsgRNA towards glpR in Pseudomonas putida
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-phaZ
Plasmid#169874PurposeSingle guide RNA plasmid for Cas9 targeting phaZ in P. putidaDepositorInsertsgRNA towards phaZ in Pseudomonas putida
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCK255
Plasmid#171155PurposeExpression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmidDepositorInsertI-SceI_sacB
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSH424-ssr
Plasmid#198026PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Sm resistance cassette
UseTagsExpressionBacterialMutationPromoterSmR promoterAvailabilityAcademic Institutions and Nonprofits only -
pSH824-ssr
Plasmid#198027PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Apr resistance cassette
UseTagsExpressionBacterialMutationPromoterAprR promoterAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2-WPRE
Plasmid#218871PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseAAVTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterGFAPAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2n-WPRE
Plasmid#218872PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAVTagsExpressionMammalianMutationS99A F104H R168PPromoterGFAPAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-iGABASnFR2-WPRE
Plasmid#218874PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseAAVTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterSynapsinAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-iGABASnFR2n-WPRE
Plasmid#218875PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAVTagsExpressionMammalianMutationS99A F104H R168PPromoterSynapsinAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-iGABASnFR2-WPRE
Plasmid#218883PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseAAVTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-iGABASnFR2n-WPRE
Plasmid#218884PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAVTagsExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pHC01
Plasmid#106900PurposeRatiometric gas biosensor plasmid for 3-oxo-C12-HSL sensingDepositorInsertsLasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+7U
Plasmid#149393PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation7bp inserted upstream, and 10bp inserted downstre…PromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+10U
Plasmid#149394PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation10bp inserted upstream and downstream of the IHFp…PromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Mut
Plasmid#149396PurposeP. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHFDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHFprox site is mutated at key positions needed f…PromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_motif_scram
Plasmid#149397PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationUpstream motif recognized by Cas1-2/3 during spac…PromoterpBADAvailabilityAcademic Institutions and Nonprofits only
Showing: 141 - 160 of 591 results