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Plasmid#218886PurposeBacterial expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseTags6xHisExpressionBacterialMutationS99A F104H R168PPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB4A3-PBAD-phzSM
Plasmid#165620PurposeArabinose-inducible expression of phzSM for conversion of phenazine-1-carboxylic acid to pyocyaninDepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilB113*Q119A
Plasmid#162867PurposeExpression of PilB Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationQ113Amber, Q119APromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilB113*L197D
Plasmid#162868PurposeExpression of PilB Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationQ113Amber, L193DPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJM253 (miniCTX1-rhaSR-PrhaBAD)
Plasmid#110571PurposeIntegration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-lvaA
Plasmid#106397PurposeGuide RNA plasmid targeting lvaA on BBR1-UP originDepositorInsertsgRNA towards lvaA in Pseudomonas putida
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB1
Plasmid#62547Purposebroad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.DepositorInserteGFP
UseBroad host range plasmidTagsExpressionBacterialMutationPromoterpTrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJM251 (miniCTX1-araC-ParaBAD)
Plasmid#110567PurposeIntegration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
UseTagsExpressionBacterialMutationPromoteraraC-ParaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pStrepIHFb
Plasmid#149385PurposeExpression of P. aeruginosa PA14, StrepII-HRV3C-IHFbetaDepositorInsertPseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
UseTagsHRV3C cleavage site and StrepII-tag withExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pchG WT
Plasmid#87107Purposeexpresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosaDepositorInsertpchG WT
UseTagshis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP119T
Plasmid#98251PurposePseudomonas T3SS gateway vector with HA-sfGFP11-sfGFP11DepositorInsertGateway cassettee-HA-sfGFP11-11
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET29b-pvdF WT
Plasmid#187974PurposeExpresses hyrdoxyornithine transformylase PvdF from Pseudomonas aeruginosaDepositorInsertpvdF WT
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_wt
Plasmid#149386PurposeP. aeruginosa PA14 CRISPR2 locusDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2-6xHis
Plasmid#218885PurposeBacterial expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseTags6xHisExpressionBacterialMutationS99A F102Y F104Y L178SPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-iGABASnFR2-WPRE-bGH-polyA
Plasmid#218868PurposeMammalian expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA
Plasmid#218870PurposeMammalian expression of improved GABA sensor control (no change in fluorescence)DepositorInsertiGABASnFR2(no bind)
UseTagsExpressionMammalianMutationS99A F102G R168PPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA
Plasmid#218869PurposeMammalian expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseTagsExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2(no bind)-6xHis
Plasmid#218887PurposeBacterial expression of improved GABA sensor control (no change in fluorescence)DepositorInsertiGABASnFR2(no bind)
UseTags6xHisExpressionBacterialMutationS99A F102G R168PPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)
Plasmid#110572PurposeIntegration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insertDepositorInsertrhaSR-PrhaBAD-stRBS-aacC1
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH824-ssr
Plasmid#198027PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Apr resistance cassette
UseTagsExpressionBacterialMutationPromoterAprR promoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only