-
Plasmid#174554PurposeAll-in-one prime editor piggyBac transposon, NG variantDepositorInsertSpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationL1111R, G1218R, E1219F, A1322R, R1335V, T1337RPromoterCAGAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PE VQR
Plasmid#162797PurposeAll-in-one prime editor piggyBac transposon, VQR variantDepositorInsertSpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationD1135V, R1335Q, T1337RPromoterCAGAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TET-PH-lox66FRT
Plasmid#139561PurposepiggyBac expression vector with TET-ON system and puromycin selectionDepositorInsertPGK-puroR-pA cassette, inserted in PB-TET vector
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TET-ZH-lox66FRT
Plasmid#139563PurposepiggyBac expression vector with TET-ON system and Zeocin selectionDepositorInsertPGK-ZeoR-pA cassette, inserted in PB-TET vector
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TET-BH-lox66FRT
Plasmid#139562PurposepiggyBac expression vector with TET-ON system and Blasticidin selectionDepositorInsertPGK-BSD-pA cassette, inserted in PB-TET vector
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEP163
Plasmid#137881PurposePiggyBac donor vector for expression of mRFP-T2A-BlasticidinRDepositorInsertmRFP-T2A-BlasticidinR
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-sfGFP-DsRed
Plasmid#80811PurposeVector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsertsfGFP-piggyBac-DsRed
UseUnspecifiedTagssfGFPExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-2xHA-DsRed
Plasmid#80822PurposeVector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert2xHA-piggyBac-DsRed
UseUnspecifiedTags2xHAExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-3xFLAG-DsRed
Plasmid#80820PurposeVector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xFLAG-piggyBac-DsRed
UseUnspecifiedTags3xFLAGExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pb-TRE3G-TYR-HygR
Plasmid#195508Purposeinducible Piggybac vector expressing human TYR CDS with hygromycin selectionDepositorInsertTYR (TYR Human)
UsePiggybacTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pb459-mU6-sgRNA-EF1a-PuroR
Plasmid#195507Purposepiggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sitesDepositorInsertPuromycin
UseCRISPR; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB Neo* PB
Plasmid#138413PurposeMinimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; PiggybacTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-DsRed-w+
Plasmid#80801PurposeVector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.DepositorInsertspiggyBac-3xP3-DsRed
GMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and GMR::hsp70Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
-
C278
Plasmid#174356PurposeFor introducing multiple copies of OsTIR1 with the piggyBac systemDepositorInsertOsTIR1
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB PUb-integrase
Plasmid#183966PurposepiggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent markerDepositorInsertPhiC31 integrase
UsePiggybac insect transgenesis destination vectorTagsExpressionMutationPromoterAedes aegypti PolyubiquitinAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PBCAG-eGFPt2amRFP
Plasmid#41001DepositorInsertGFPt2a
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PBCAG-mRFP
Plasmid#40996DepositorInsertmRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PBCAG-Cerulean
Plasmid#40997DepositorInsertCerulean
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only