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Plasmid#15863DepositorInsertH2-Q1 (H2-Q1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
GB1-CEP55(160-217, S215C)
Plasmid#186793PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CEP55(160-217, S215C)DepositorInsertGB1-His6-TEV-CEP55(160-217, S215C) (CEP55 Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS215CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)
Plasmid#199242PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS191C, I225A, L228A, W231APromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4B(S184C)
Plasmid#180026PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).DepositorInsertGB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS184CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSKB2-TRex
Plasmid#31349DepositorInsertThermus aquaticus Redox Sensing Repressor
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
V2rf2-IRES-BL Targeting Vector
Plasmid#15860DepositorInsertV2rf2 (Vmn2r81 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
V2rf2 Southern Blot 5'-external probe
Plasmid#15862DepositorInsertV2rf2 (Vmn2r81 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pISH-bovine-tau
Plasmid#15864DepositorInsertbovine tau (MAPT )
UseTagsExpressionMutationPromoterAvailable sinceMay 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfCSF3b
Plasmid#65613Purposeexpress soluble His-tagged zebrafish CSF3b/Gcsfb in E. coliDepositorInsertCsf3b (csf3b Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-GFP
Plasmid#127888PurposeProvides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP taggingDepositorInsertPuro-GFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-mCherry
Plasmid#127887PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry taggingDepositorInsertPuro-mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-HBTH
Plasmid#127883PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH taggingDepositorInsertGFP-Puro-HBTH
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-H3FAID
Plasmid#127884PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID taggingDepositorInsertGFP-Puro-H3F-AID
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-HF
Plasmid#127882PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F taggingDepositorInsertGFP-Puro-H3F
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
dFP
Plasmid#120542PurposeExpresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.DepositorInsertde novo fluorescence-activating protein (dFP)
UseTags6xHis - TEV cleavageExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSKB2-TRexR90D
Plasmid#31350DepositorInsertThermus aquaticus Redox Sensing Repressor (R90D)
UseTagsHisExpressionBacterialMutationchanged Arginine 90 to Aspartic AcidPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
dFP-mini
Plasmid#120543PurposeExpresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.DepositorInsertde novo fluorescence-activating protein mini (dFP-mini)
UseTags6xHis - TEV cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-3XHA
Plasmid#127881PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA taggingDepositorInsertGFP-Puro-3XHA (PPP2CA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only