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pLVX-NPM1wt-IRES-mCherry

Plasmid

#131824

Purpose

Lentiviral plasmid expressing NPM1 wildtype (human) in mammalian cells upon transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-4 dCARD

Plasmid

#208785

Purpose

Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cry2 mCherry Caspase4 dCARD (CASP4 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

CARD domain is removed

Promoter

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-P112H

Plasmid

#133760

Purpose

Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed proline 112 to histidine.

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-dL5**-mCER-TRF1

Plasmid

#168176

Purpose

Expresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1

Depositor

Patricia Opresko

Article

Fouquerel et al Mol Cell. 2019 Jul 11;75(1):117-130.e6.

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

FAP (dL5**) and mCerulean

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-i3 ONE-GO

Plasmid

#204329

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-i3 ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Gβγ-BERKY3

Plasmid

#158426

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-HA-UBE3B-IRES-Puro

Plasmid

#107345

Purpose

Expresses HA tagged UBE3B in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-oB ONE-GO

Plasmid

#189733

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-oB ONE-GO
GNAO (Gnao1 Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A382T

Plasmid

#133756

Purpose

Lentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

Changed alanine 382 to threonine

Promoter

CMV

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-s ONE-GO

Plasmid

#204327

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-s ONE-GO (GNAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-q ONE-GO

Plasmid

#204331

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-q ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-NPM1c-IRES-mCherry

Plasmid

#131825

Purpose

Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOneZeo-ORF18(WT)-3xFLAG

Plasmid

#129746

Purpose

Dox inducible expression of KSHV ORF18-3xFLAG in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

TRE3Gs and Tet inducible promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-UBE3B-HA-IRES-Puro

Plasmid

#107346

Purpose

Expresses HA tagged UBE3B in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-NLS-HA-TurboID-PCNA

Plasmid

#215076

Purpose

Expresses NLS-HA-TurboID-PCNA in mammalian cells from a lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Lentiviral

Tags

NLS-HA-TurboID

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-tdTomato-FlagAkt1W80A

Plasmid

#64833

Purpose

cDNA of Akt1 with point mutation from Trp to Ala at position 80. This mutation confers resistance to the MK-2206 drug

Depositor

Eva Gonzalez

Article

Kajno et al Biochem J. 2015 Apr 9.

Insert

Akt1 (Akt1 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Point mutation from Trp to Ala at position 80

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-Puro-mRuby-Sec23A

Plasmid

#36158

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec23A (SEC23A Human)

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES Hyg SMAD4

Plasmid

#107128

Purpose

Constitutive Flag-tagged SMAD4 expression in Mammalian cells

Depositor

Joan Massague

Article

David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX Tight Puro Snail

Plasmid

#101117

Purpose

Tet-On Snail

Depositor

Joan Massague

Article

David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

Insert

Snail cDNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Gαq*-BERKY3

Plasmid

#158425

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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