pLVX-IRES-Gαq*-BERKY3
(Plasmid
#158425)
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PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158425 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX-IRES-Hyg
- Backbone size w/o insert (bp) 8519
- Total vector size (bp) 12073
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Vector typeLentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
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Alt nameGαq*-BERKY3
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Insert Size (bp)3554
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-IRES-Gαq*-BERKY3 was a gift from Mikel Garcia-Marcos (Addgene plasmid # 158425 ; http://n2t.net/addgene:158425 ; RRID:Addgene_158425) -
For your References section:
Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor Design. Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, Garcia-Marcos M. Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 10.1016/j.cell.2020.06.020. 10.1016/j.cell.2020.06.020 PubMed 32634377