Addgene

We narrowed to 3,652 results for: max

Showing: 141 - 160 of 3652 results

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iBE4max YE1 NG CLYBL

Plasmid

#174570

Purpose

doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

tet ON

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-FLuc

Plasmid

#188311

Purpose

Expression of Firefly luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Firefly luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Mutation

Promoter

T7Max

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-YE1-BE4max

Plasmid

#198546

Purpose

Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C-BE4max

Plasmid

#183679

Purpose

Expresses eNme2-C-BE4 in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

rAPOBEC1-neNme2-C-2xUGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-RLuc

Plasmid

#188312

Purpose

Expression of Renilla luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Renilla luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Mutation

Promoter

T7Max

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAC93-pmax-dCas9VP160

Plasmid

#48225

Purpose

dCas9VP160 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR

Tags

HA Tag and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABEmax-SpRY

Plasmid

#195279

Purpose

Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

Depositor

Carsten Lederer, Petros Patsali

Insert

human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAC90-pmax-DEST

Plasmid

#48222

Purpose

Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR; Gateway destination

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4/HisMaxB-YAP1

Plasmid

#18978

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pTH733-2µ-RLuc/maxCFLuc

Plasmid

#40608

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pTY137 psfGFP-bimax2

Plasmid

#202410

Purpose

Expresses sfGFP-bimax2 to inhibit importin-alpha-mediated nuclear import

Depositor

Tim Mitchison

Article

Yoo et al J Cell Biol. 2021 Jul 5;220(7). pii: 212

Insert

bimax2

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAC91-pmax-dCas9VP64

Plasmid

#48223

Purpose

dCas9VP64 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP64 activation domain

Use

CRISPR

Tags

HA Tag and VP64

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max-CP1028

Plasmid

#138160

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-BE3.9max C-terminal

Plasmid

#137172

Purpose

Expresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-BE3.9max_C-terminal

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-BE3.9max N-terminal

Plasmid

#137171

Purpose

Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-BE3.9max_N-terminal

Use

Tags

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAT15547_PEmax-SuperFi-Cas9

Plasmid

#184377

Purpose

Mammalian expression of SuperFi-Cas9 PEmax prime editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

PEmax-SuperFi-Cas9

Use

CRISPR

Tags

SV40 NLS and c-myc NLS

Expression

Mammalian

Mutation

H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-ABEmax C-terminal

Plasmid

#137174

Purpose

Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-ABEmax_C-terminal

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-ABEmax N-terminal

Plasmid

#137173

Purpose

Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-ABEmax_N-terminal

Use

Tags

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1804_pmax-dCasRx-RBM38

Plasmid

#118638

Purpose

Transient transfection; Expresses dCasRx-RBM38; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-RBM38

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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