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Plasmid#58928PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-272aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP9B(E272Q)-HA
Plasmid#209257PurposeMammalian expression of ATP9B mutant E272QDepositorInsertATP9B (ATP9B Human)
UseRetroviralTagsHAExpressionMutationE272QPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-K272A
Plasmid#222215PurposeExpress DDX53 mutant K272A protein in bacteriaDepositorInsertDDX53_K272A (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationK272APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX53-K272A
Plasmid#222242PurposeExpress FLAG tagged DDX53 mutant K272A in mammalian cellsDepositorInsertDDX53 (DDX53 Human)
UseTags3xFLAGExpressionMammalianMutationK272APromoterT7Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_EXOSC6:1-272
Plasmid#211084PurposeMammalian expression of full-length human EXOSC6. C-terminal NanoLuc luciferase tag.DepositorInsertEXOSC6:M1-P272 (EXOSC6 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC272-P3G8
Plasmid#203113PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC272DepositorInsertEGFP/BC272
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP9B(E272Q)-HA
Plasmid#209245PurposeMammalian expression of ATP9B mutant E272QDepositorInsertATP9B (ATP9B Human)
UseTagsHAExpressionMammalianMutationE272QPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-IRG1-L272Q-HA
Plasmid#198184PurposeMammalian expression of HA-tagged human ACOD1 (IRG1) L272QDepositorInsertACOD1 (ACOD1 Human)
UseTagsHAExpressionMammalianMutationThe leucine in IRG1 at position 272 mutates to gl…PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A]
Plasmid#188594PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-Flag-GK (12-272)
Plasmid#134272PurposeLentivector encoding Flag-tagged GK truncation (amino acids 12-272)DepositorInsertGk (Gk Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk amino acids 12-272PromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSV272-PfMBP-HypDAE
Plasmid#110296PurposeExpresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3)DepositorInsertt-4-Hydroxy-L-proline Dehydratase Activating Enzyme
UseTagsHis6 and Maltose Binding ProteinExpressionBacterialMutationPromoterT7Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
YCe2725 HC_Kan_conn_BsaI-2_p24
Plasmid#100669PurposeEMMA toolkit Assembly connector for second step assemblyDepositorInsertEMMA toolkit Assembly connector for second step assembly
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATP23 gRNA (BRDN0001148272)
Plasmid#77436Purpose3rd generation lentiviral gRNA plasmid targeting human ATP23DepositorInsertATP23 (XRCC6BP1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
UHMK1 gRNA (BRDN0001162272)
Plasmid#77182Purpose3rd generation lentiviral gRNA plasmid targeting human UHMK1DepositorInsertUHMK1 (UHMK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-Hsp90 M(272-617)
Plasmid#22482PurposePlease note this plasmid contains GST-Hsp90 residues 273-617.DepositorInsertHsp90alpha M domain (HSP90AA2P Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(272)
Plasmid#44375DepositorInsertUAS1B16-TEF(272) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(272)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
p6272 pDEST-60-GST-HPV16E6
Plasmid#24127DepositorUseTagsExpressionBacterial and MammalianMutationnonePromoterAvailable sinceApril 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(272)
Plasmid#44376DepositorInsertUAS1B8-TEF(272) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(272)Available sinceApril 16, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
R777-E272 Hs.RB1-nostop
Plasmid#70556PurposeGateway ORF clone of human RB1 [NM_000321.2] without stop codon (for C-terminal fusions)DepositorInsertRB1 (RB1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXL-CAG-mBirA272-NRX3b
Plasmid#43921DepositorInsertBirA272-NRX3b (birA Synthetic, Human)
UseTags2XFLAGExpressionMammalianMutationSee commentsPromoterCAGAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only