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Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA
Plasmid#174900Purposeamber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertCRFR1 (Crhr1 Rat)
UseTagsHAExpressionMammalianMutationA95TAGPromoterEF1Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Syn-ATP
Plasmid#51819PurposeOptical reporter of presynaptic ATPDepositorInsertChimeric Synaptophysin-mCherry-Luciferase
UseTagsExpressionMammalianMutationWithin WT luciferase gene, changed Threonine 214 …PromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-KIF1A
Plasmid#102850PurposeOverexpresses KIF1A and cell fill TdTomatoDepositorInsertKIF1A (Kif1a Rat)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV mRuby3-Synapsin1a
Plasmid#187896PurposeFor labeling pre-synaptic terminalsDepositorInsertSynapsin1a
UseTagsmRuby3ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-mDlx-jGCaMP8m-WPRE
Plasmid#176754PurposejGCaMP8m expression in GABA-ergic interneurons under the control of mDlx enhancerDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromotermDlxAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myo 9a-GFP
Plasmid#134959PurposeExpression of rat Myo 9a (Myr7) in mammalian cells.DepositorInsertMyo 9a (rat) with 5' UTR (Myo9a Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Halo-Tag-128-425-Akap9
Plasmid#196873PurposeExpression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from GolgiDepositorInsertHalo-Tag-Akap9 (128-425) (Akap9 Rat)
UseTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-mDlx-jGCaMP8s-WPRE
Plasmid#176755PurposejGCaMP8s expression in GABA-ergic interneurons under the control of mDlx enhancerDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromotermDlxAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK289 (mAID-mClover-NeoR)
Plasmid#72827PurposeC-terminal tagging with mAID-mClover using CRISPR/Cas9DepositorInsertmAID-mClover-Neo
UseCRISPRTagsmAID-mCloverExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt4
Plasmid#184505PurposeMammalian expression of Synaptotagmin 4 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 4 (Syt4 Rat)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5/G226A/A366S)
Plasmid#55797PurposeThis highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.DepositorInsertalpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T, G226A, A366S i…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-Ftractin-mCherry
Plasmid#85131Purposelentiviral expression of an F-actin live-cell marker (mCherry)DepositorInsertFtractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
UseLentiviralTagsmCherryExpressionMutationPromoterEF1aAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-Homer1-C-18
Plasmid#57279PurposeLocalization: Membrane Adaptor, Excitation: 503, Emission: 518DepositorInsertHomer1 (Homer1 Rat)
UseTagsDronpaExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-CamKII-TRPV1-P2A-mCherry
Plasmid#200829PurposeTransduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporterDepositorInsertTRPV1-P2A-mCherry (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
ISceI-GR-RFP
Plasmid#17654DepositorUseTagsmRFPExpressionMammalianMutationC655G mutation in the glucocorticoid receptor (se…PromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
mCit-4aa-SNAP25B
Plasmid#53235PurposeFluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Lysosomes-20
Plasmid#55073PurposeLocalization: Lysosome Membrane, Excitation: 587, Emission: 610DepositorInsertLAMP1
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-bJunVN173
Plasmid#22012DepositorInsertbJunVN173 (Jun Rat, Aequorea Victoria)
UseTagsFLAGExpressionMammalianMutationc-Jun(257-338)-Venus(1-172)PromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Camk2a KI
Plasmid#131484PurposeEndogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only