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Plasmid#40132PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).DepositorTypeEmpty backboneUseTagsFokI nuclease (ELD)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only
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pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination
Plasmid#40131PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).DepositorTypeEmpty backboneUseTagsFokI nuclease (KKR)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-eSpCAS9-NG-pA
Plasmid#131466PurposeExpresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized eSpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJS124
Plasmid#207593PurposeGFP-BIB H20A expressionDepositorInsertGFP-BIB H20A
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2_8xCTS-ECFP
Plasmid#200249PurposeZebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesisDepositorInsert8xCTS-ECFP
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c013
Plasmid#175450PurposeProtein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220.DepositorInsertCD44 (CD44 Human)
UseTagsHis6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-SpCAS9-NG-pA
Plasmid#131465PurposeExpresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized SpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0553
Plasmid#128989PurposeLEU2 integration vector of pZF(3BS)-mRUBY2DepositorInsertLeu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_dTK
Plasmid#133244PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertdrosophila tachykinin-fused codon-optimized GCaMP6s
UseTagsExpressionInsectMutationPromoterhsp70Available sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mcGAS-LL/RK-NLS
Plasmid#185457PurposeInducible nuclear cGASDepositorInsertcGAS
UseLentiviralTagsFLAG and SV40 NLSExpressionMammalianMutationL157R, L159KPromoterAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mcGAS-LL/RK-NLS/D307A
Plasmid#185459PurposeInducible nuclear cGAS with D307A mutationDepositorInsertcGAS
UseLentiviralTagsFLAG and SV40 NLSExpressionMammalianMutationL157R, L159K, D307APromoterAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis -MH)
Plasmid#195613PurposeExpresses the wild-type I-SceI homing endonuclease from the T7 promoterDepositorInsertI-SceI
UseTagsExpressionBacterialMutationNonePromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a D832A
Plasmid#113517PurposeExpresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nucleaseDepositorInsertLbCas12a
UseTagsMBPExpressionBacterialMutationD832APromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJS96
Plasmid#207588PurposeGFP-TonBH20A expressionDepositorInsertGFP-TonBH20A
UseTagsExpressionBacterialMutationH20APromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmOrange 2-Actin
Plasmid#199703Purposevisualization of cleavage furrow in living mammalian mitotic cellsDepositorInserthuman cytoplasmic action
UseTagsmOrange 2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6Z-806
Plasmid#85255PurposePlasmid clone containing HHV-6B(Z29) restriction endonuclease fragmentDepositorInsertfragment of the human herpesvirus 6B strain Z29 genome
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM0530
Plasmid#128987PurposePart plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) partDepositorInsertSV40NLS-ZiF268DBD-CRY2PHR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL8_3xG34-DCAF8L2_X1
Plasmid#191519PurposeExpression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)DepositorInsertZF-DdCBE 3xG34-DCAF8L2 X1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL5_3xG22-DCAF8L2_X1
Plasmid#191516PurposeExpression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)DepositorInsertZF-DdCBE 3xG22-DCAF8L2 X1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -