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pJN105-Hygro-GFP

Plasmid

#41883

Depositor

Howard Steinman

Article

Bandyopadhyay et al Infect Immun. 2004 Aug;72(8):4541-51.

Insert

Ptac::GFP-mut-2

Use

Bacterial broad host range vector

Tags

Expression

Bacterial

Mutation

GFP-mut-2 is GFP with Ser65Ala, Val68Leu, and Ser…

Promoter

Ptac

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only
pENTR1A-NTAP-A (w322-1)

Plasmid

#17402

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR221-VPS35

Plasmid

#166220

Purpose

Gateway entry vector for N. furzeri VPS35

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al bioRxiv

Insert

VPS35

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G12R

Plasmid

#83143

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G12R

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR1A-FKBP12DD C-term (w350-2)

Plasmid

#17413

Purpose

Gateway entry vector for Degron, C-term

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

FKBP12DD (L106P)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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p5E-QUASM1

Plasmid

#155114

Purpose

5' entry vector containing QUASM1 promoter element.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1

Use

Gateway 5' entry vector

Tags

Expression

Mutation

Promoter

QUASM1-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2

Plasmid

#25752

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pmKalama1-Nuc

Plasmid

#14894

Purpose

Mammalian expression of mKalama1 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 Apr 20. ():.

Insert

pmKalama1-Nuc

Use

Tags

NLS

Expression

Mammalian

Mutation

I178T mismatch, replace EYFP of the vector pEYFP-…

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pENTR/pSM2(CMV) GFP (w513-1)

Plasmid

#19170

Purpose

Gateway entry vector for miRNA expression under CMV promoter and GFP reporter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi; Entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR

Plasmid

#109055

Purpose

Bacterial expression of E.coli DHFR protein

Depositor

Stephen Benkovic

Article

Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b Q61L

Plasmid

#83134

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Q61L

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_E2-Crimson

Plasmid

#62410

Purpose

MXS_chaining vector with E2-Crimson

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

E2-Crimson

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G13C

Plasmid

#83145

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13C mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G13C

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b Q61R

Plasmid

#83135

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Q61R

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-EOS-GFP-IP

Plasmid

#60439

Purpose

PiggyBac for EOS-GFP

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

EOS

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pABY-c46

Plasmid

#201774

Purpose

Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Use

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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p415 Glo3

Plasmid

#112651

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3

Use

Tags

Expression

Yeast

Mutation

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR/pSM2(CMV) (w57-1)

Plasmid

#17389

Purpose

Gateway entry vector for miRNA expression under CMV promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi; Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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YIPlac204TKC-E2-Crimson-HDEL

Plasmid

#38771

Depositor

Benjamin Glick

Article

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Use

Tags

Linker peptide and yeast ER retention signal sequ…

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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