p5E-QUASM1
(Plasmid
#155114)
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Purpose5' entry vector containing QUASM1 promoter element.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 155114 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONRP4-P1R
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2643
- Total vector size (bp) 2880
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Vector typeGateway 5' entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameQUASM1
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SpeciesSynthetic
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Insert Size (bp)237
- Promoter QUASM1-E1b
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer CAC GAC GTT GTA AAA CGA C
- 3′ sequencing primer CAG GAA ACA GCT ATG AC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Can be recombined with pME and p3E vectors to make a QFGal4-dependent reporter construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5E-QUASM1 was a gift from Bret Pearson (Addgene plasmid # 155114 ; http://n2t.net/addgene:155114 ; RRID:Addgene_155114) -
For your References section:
An optimized QF-binary expression system for use in zebrafish. Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ. Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 10.1016/j.ydbio.2020.07.007. 10.1016/j.ydbio.2020.07.007 PubMed 32697972