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Plasmid#37198DepositorInsertFokI (fused to Rosa L6 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs L6, and SV40 NLSExpressionMammalianMutationQ486E, I499L and N496DPromoterCMVAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab6WT
Plasmid#46781Purposeexpression of WT Rab6a in mammalian cellsDepositorInsertRab6 (RAB6A Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 HA-TBRII (delta Cyt)
Plasmid#14051DepositorInsertTGF beta rec II (delta cyt) (TGFBR2 Human)
UseTagsHA (PYDVPDYA)ExpressionMammalianMutationDominant negative mutant. A stop codon was introd…PromoterAvailable sinceMay 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GFP-MHC-IIB
Plasmid#35691DepositorInsertMHC IIB (MYH10 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-MAGE-2-429
Plasmid#89090Purposemammalian expression of full-length human MAGE-A11 with N-terminal FLAG tagDepositorInsertMAGE-A11 (MAGEA11 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV/myc/mito-hOGG1
Plasmid#18706DepositorInsert8-oxoguanine DNA glycosylase (OGG1 Human)
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SARS-CoV-2 Nsp3TMC111A
Plasmid#204207PurposeMammalian expression of SARS-CoV-2 Nsp3DepositorInsertSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
UseTagsFLAGx1ExpressionMammalianMutationCys to Ala in active site of PLproPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-STING-C1471S-FLAG
Plasmid#198188PurposeMammalian expression of FLAG-tagged mouse STING1 C1471SDepositorInsertTMEM173 (Sting1 Mouse)
UseTagsFLAGExpressionMammalianMutationThe cysteine in STING at position 147 mutates to …PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-3xFLAG-prs-MCP1
Plasmid#184341PurposeExpress yeast MCP1 in Expi293F cellsDepositorInsertYeast MCP1 (MCP1 Budding Yeast)
UseTags3xFLAG-PreScission cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN beta
Plasmid#40929DepositorInsertEPLIN beta (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SARS-CoV-2 Nsp3TM
Plasmid#204206PurposeMammalian expression of SARS-CoV-2 Nsp3DepositorInsertSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
UseTagsFLAGx1ExpressionMammalianMutationnone, from obtained clonePromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3-Y130F
Plasmid#113428PurposeExpresses hA3A-BE3-Y130F in mammalian cellsDepositorInserthA3A-BE3-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRTagsExpressionMammalianMutationhAPOBEC3A_Y130FPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-Flag-Smurf1 C699A
Plasmid#11753DepositorInsertSMURF1 (SMURF1 Human)
UseTagsFlagExpressionMammalianMutationC699A: catalytically inactivePromoterAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcmv3-STING-C88S-FLAG
Plasmid#198187PurposeMammalian expression of FLAG-tagged mouse STING1 C88SDepositorInsertTMEM173 (Sting1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B Flag BimEL
Plasmid#23090DepositorInsertBim EL (Bcl2l11 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PARP1-3xFlag-7xKQ
Plasmid#111576PurposeExpresses full length PARP1 with K to Q mutations in automodification domain in mammalian cellsDepositorInsertPARP1 (PARP1 Human)
UseTags3xFlagExpressionMammalianMutationlysine to glutamine mutation at the following ami…PromoterCMVAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV human IRS-1 CAAX
Plasmid#11238DepositorInsertIRS-1 (IRS1 Human)
UseTagsCAAXExpressionMammalianMutationC-terminal prenylation motif makes IRS-1 localize…PromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-Flag-Smurf2 C716A
Plasmid#11747DepositorInsertSMURF2 (SMURF2 Human)
UseTagsFlagExpressionMammalianMutationC716A: catalytically inactivePromoterAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-erHRP(N175S mutant)
Plasmid#79909PurposeerHRP(N175S mutant) pCMV backboneDepositorInserterHRP(N175S mutant)
UseTagsHA tagExpressionMammalianMutationN175S mutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
3XFlag pCMV5-TOPO TAZ ∂CC
Plasmid#24816DepositorInsertTAZ (WWTR1 Human)
UseTags3XFlagExpressionMammalianMutationDeletion of amino acids 222-265 (coiled coil doma…PromoterAvailable sinceMay 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits